DAPK2 Antibody (X15): sc-100371
- DAPK2 Antibody (X15) is a mouse monoclonal IgG1 κ DAPK2 antibody provided at 100 µg/ml
- raised against recombinant DAPK2 of human origin
- recommended for detection of DAPK2 of human origin by WB, IP and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for DAPK2 Antibody (X15). This work is in progress.
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DAPK2 Antibody (X15) is a mouse monoclonal IgG1 kappa light chain antibody that detects DAPK2 protein of human origin by western blotting (WB), immunoprecipitation (IP), and enzyme-linked immunosorbent assay (ELISA). Anti-DAPK2 antibody (X15) is available as the non-conjugated format. Death-associated protein kinase 2 (DAPK2), also known as death-associated protein kinase-related protein-1 (DRP-1), is a calcium/calmodulin-regulated serine/threonine kinase that plays a crucial role in cellular signaling pathways. DAPK2 binds to calmodulin and undergoes autophosphorylation in response to elevated intracellular calcium levels, which is essential for kinase activity. DAPK2 is primarily localized in the cytoplasm and is expressed in various tissues, including the heart, lung, and skeletal muscle. DAPK2′s ability to phosphorylate myosin light chain (MLC) as an exogenous substrate is significant for regulating cytoskeletal dynamics and cell motility. Furthermore, DAPK2 exhibits considerable homology to DAP-kinase, a protein known to mediate interferon (IFN)-γ-induced apoptosis in HeLa cells, suggesting a role in apoptosis and tumor suppression. Given DAPK2′s involvement in these critical processes, DAPK2 is considered a potential tumor suppressor gene, making DAPK2 an important target for research in cancer biology and therapeutic development.
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DAPK2 Antibody (X15) References:
- A death-associated protein kinase (DAPK)-interacting protein, DIP-1, is an E3 ubiquitin ligase that promotes tumor necrosis factor-induced apoptosis and regulates the cellular levels of DAPK. | Jin, Y., et al. 2002. J Biol Chem. 277: 46980-6. PMID: 12351649
- DAPK catalytic activity in the hippocampus increases during the recovery phase in an animal model of brain hypoxic-ischemic injury. | Schumacher, AM., et al. 2002. Biochim Biophys Acta. 1600: 128-37. PMID: 12445468
- Frequent promoter methylation of CDH1, DAPK, RARB, and HIC1 genes in carcinoma of cervix uteri: its relationship to clinical outcome. | Narayan, G., et al. 2003. Mol Cancer. 2: 24. PMID: 12773202
- Expression of a novel type I keratin, DAPK-1 in the dorsal aorta and pronephric duct of the zebrafish embryos. | Jang, WS., et al. 2003. Gene. 312: 145-50. PMID: 12909350
- Promoter methylation and down-regulation of DAPK is associated with gastric atrophy. | Kim, WS., et al. 2003. Int J Mol Med. 12: 827-30. PMID: 14612952
- Bidirectional signals transduced by DAPK-ERK interaction promote the apoptotic effect of DAPK. | Chen, CH., et al. 2005. EMBO J. 24: 294-304. PMID: 15616583
- Assignment of DAP1 and DAPK--genes that positively mediate programmed cell death triggered by IFN-gamma--to chromosome regions 5p12.2 and 9q34.1, respectively. | Feinstein, E., et al. 1995. Genomics. 29: 305-7. PMID: 8530096
- DAP kinase links the control of apoptosis to metastasis. | Inbal, B., et al. 1997. Nature. 390: 180-4. PMID: 9367156
- ZIP kinase, a novel serine/threonine kinase which mediates apoptosis. | Kawai, T., et al. 1998. Mol Cell Biol. 18: 1642-51. PMID: 9488481
- Molecular cloning and developmental expression of a rat homologue of death-associated protein kinase in the nervous system. | Sakagami, H. and Kondo, H. 1997. Brain Res Mol Brain Res. 52: 249-56. PMID: 9495546
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAPK2 Antibody (X15) | sc-100371 | 100 µg/ml | $333.00 |