DAPI CAS: 28718-90-3
MF: C16H15N5•2HCl
MW: 350.24

DAPI (CAS 28718-90-3)

DAPI | CAS 28718-90-3 is rated 4.9 out of 5 by 11.
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Alternate Names: DAPI also known as 4′,6-Diamidino-2-phenylindole Hydrochloride; DIHYDROCHLORIDE; 4′,6-DIAMIDINO-2-PHENYLINDOLE, 2HCL;4′,6-DIAMIDINO-2-PHENYLINDOLE DIHYDROCHLORIDE; 4′,6-DIAMIDINO-2-PHENYLINDOLE, HYDROCHLORIDE; 4′,6-DIAMIDINE-2′-PHENYLINDOLE DIHYDROCHLORIDE; 2-(4-AMIDINOPHENYL)-6-INDOLECARBAMIDINEDIHYDROCHLORIDE; 2-phenylindole-4′,6-dicarboxamidine dihydrohydrochloride (hydrate)
Application: DAPI® is a cell-permeable DNA-binding dye preferential to adenine and thymine rich DNA and can be used for flow cytometry
CAS Number: 28718-90-3
Purity: ≥97%
Molecular Weight: 350.24
Molecular Formula: C16H15N52HCl
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

DAPI is a cell-permeable DNA-binding dye which binds preferentially to DNA rich in adenine and thymine. DAPI is used in flow cytometry for measuring nuclear DNA content or for sorting isolated chromosomes and is also useful for microscopic detection of nuclei and nuclear DNA in normal and apoptotic cells. DAPI can also be used to detect mycoplasma and in simple retrograde double-labelling procedures for studying axonal branching employing Evans Blue and Primulin. DAPI is a reversible inhibitor of S-adenosyl-L-methionine decarboxylase and KAO (diamine oxidase).


References

1. Cubria, C., et al. 1994. Comp. Biochem. Physiol. C. 105: 251-254. PMID: 8103731

2. Hotz, M.A., et al. 1994. Cytometry. 15: 237-244. PMID: 8187583

3. Lee, L.S., et al. 1993. J. Biochem. Biophys. Methods. 26: 249-260. PMID: 8409197

4. Miller, T., et al. 1993. BioTechniques. 15: 1042-1047. PMID: 8292336

5. Gray, J.W., et al. 1982. Science. 238: 323-329. PMID: 2443974

6. Hessling, J.J., et al. 1980. J. Immunol. Methods. 38: 315-324. PMID: 7003019

Usage :
Working concentration: 1.5µg/ml
Physical State :
Solid
Solubility :
Soluble in water (10 mg/ml,warm), methanol, ethanol, and DMSO.
Storage :
Store at -20° C
Melting Point :
330° C (dec.)
Fluoresence :
λex ~359 nm, λem ~461 nm; λex 340 nm, λem 488 nm(nur DAPI); λex 364 nm, λem 454 nm(DAPI-DNA-Komplex:100 mM NaCl, 10 mM EDTA, 10 mM Tris, pH 7)
pK Values :
pKb: 13.73 (Predicted)
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
RTECS :
NL5995050
PubChem CID :
160166
MDL Number :
MFCD00012681
EC Number :
249-186-7
Beilstein Registry :
4894417
SMILES :
C1=CC(=CC=C1C2=CC3=C(N2)C=C(C=C3)C(=N)N)C(=N)N.Cl.Cl

Download SDS (MSDS)

Certificate of Analysis

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DAPI (CAS 28718-90-3) Product Citations

See how others have used DAPI (CAS 28718-90-3). Click on the entry to view the PubMed entry .

Citations 1 to 10 of 573 total

PMID: # 31391281  2019. mSphere. 4:

PMID: # 30615919  2019. Free Radic. Biol. Med. 134: 288-303.

PMID: # 30375708  Yoon, YJ.|Kim, YH.|Lee, YJ.|Choi, J.|Kim, CH.|Han, DC.|Kwon, BM.| et al. 2019. Cancer Sci. 110: 366-378.

PMID: # 30888336  Li, L.|Liu, Y.|Li, S.|Yang, Y.|Zeng, C.|Rong, W.|Liang, H.|Zhang, M.|Zhu, X.|Kidder, K.|Liu, Y.|Liu, Z.|Zen, K.| et al. 2019. JCI Insight. 5:

PMID: # 30521940  Chang, J.|Zhang, XL.|Yu, H.|Chen, J.| et al. 2019. Brain Res. Bull. 146: 1-6.

PMID: # 30982162  Chen, W.|Liu, H.|Wang, T.|Bao, G.|Wang, N.|Li, RC.| et al. 2019. J. Mol. Neurosci.

PMID: # 31095793  2019. J. Cell. Biochem. 120: 16643-16657.

PMID: # 31405028  2019. Cells. 8:

PMID: # 30840970  Karaoz, E.|Tepekoy, F.|Yilmaz, I.|Subasi, C.|Kabatas, S.| et al. 2019. J Korean Neurosurg Soc. 62: 153-165.

PMID: # 30475390  Cecon, E.|Ivanova, A.|Luka, M.|Gbahou, F.|Friederich, A.|Guillaume, JL.|Keller, P.|Knoch, K.|Ahmad, R.|Delagrange, P.|Solimena, M.|Jockers, R.| et al. 2019. J. Pineal Res. 66: e12540.

Citations 1 to 10 of 573 total

Hello When should i get DAPI at Nigdi, Pune.

Asked by: Pooj
For accurate availability and shipping information regarding of our chemical products, please contact our Customer Service group by phone, e-mail or live chat. Alternatively, please contact your local distributor, you can find their contact information here: https://www.scbt.com/scbt/resources/distributors
Answered by: Tech Service
Date published: 2017-06-26

We really like the DAPI, nice and bright. Does it have anti fade properties?

Asked by: Jz28sail
Thank you for your question. We are glad you have had success using sc-3598, DAPI. There are no inherent anti-fade properties in 4',6-Diamidino-2-phenylindole dihydrochloride (DAPI). We do offer two DAPI stain mounting media products which include anti-fade and anti-photobleaching properties: sc-24941, UltraCruz® Aqueous Mounting Medium with DAPI (https://www.scbt.com/scbt/product/ultracruz-mounting-medium?requestFrom=search) or sc-359850, UltraCruz® Hard-set Mounting Medium with DAPI (https://www.scbt.com/scbt/product/ultracruz-hard-set-mounting-medium?requestFrom=search).
Answered by: Chemical Support 5
Date published: 2017-04-13
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Rated 5 out of 5 by from tres bien je suis très contente d'avoir acheter ce produit!!!
Date published: 2019-10-29
Rated 5 out of 5 by from Fluorescent imaging Effectively stained the nuclei of adipose-derived stem cells. Used as a concentration of 1ug/mL
Date published: 2019-03-06
Rated 5 out of 5 by from Great product I bought this 3-4 months ago and I used it in immunofluorescence and flow cytometry assays. Very good quality.
Date published: 2016-12-08
Rated 4 out of 5 by from DAPI I have been using Dapi more than decades but recently moved to SantaCruz DAPI. works well
Date published: 2016-11-29
Rated 5 out of 5 by from Worked well Worked well, used with RAW 264.7 + LPS/IFN Cell Lysate.
Date published: 2015-09-04
Rated 5 out of 5 by from Worked well in IF Worked well in IF
Date published: 2015-09-04
Rated 5 out of 5 by from Publishable data obtained using DAPI from SCBT (sc Publishable data obtained using DAPI from SCBT (sc-3598) as a counterstain on S2 cells (PubMed ID 24603360). -SCBT Publication Review
Date published: 2015-05-11
Rated 5 out of 5 by from It has been reported (PubMed ID 20543181) It has been reported (PubMed ID 20543181) that product sc-3598 from SCBT was used successfully to counterstain mouse coronal sections prior to sealing with a coverslip. -SCBT Publication Review
Date published: 2015-01-13
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