DAP-5 Antibody (B-8): sc-137131

DAP-5 Antibody (B-8) is a mouse monoclonal IgG2a antibody that detects Death-associated protein 5 (DAP-5) in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). DAP-5 plays a crucial role in the regulation of translation, particularly during apoptosis, where DAP-5 acts as a general repressor of translation by forming inactive complexes with eukaryotic initiation factors eIF4A and eIF3, while notably lacking interaction with the CAP binding protein eIF4E. This unique mechanism allows DAP-5 to mediate cap-independent translation from its own internal ribosome entry site, creating a positive feedback loop that sustains DAP-5 expression even in the context of cellular stress. DAP-5′s ability to regulate gene expression pathways is essential for cellular differentiation, highlighting DAP-5′s importance in both normal physiological processes and in the cellular response to stress. With high conservation across species and ubiquitous expression, DAP-5 is a significant player in the intricate network of translational control, making anti-DAP-5 antibody (B-8) an invaluable tool for researchers studying apoptosis and gene regulation.

The preferred DAP-5 antibody is DAP-5 Antibody (F-2): sc-137011.