Dansyl fluoride (CAS 34523-28-9)
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Dansyl fluoride is a chemical compound employed in proteomics and enzyme research due to its fluorescent properties. It serves as a reagent for labeling primary amine groups in proteins and peptides, allowing researchers to visualize these biomolecules through fluorescence spectroscopy. This characteristic makes Dansyl fluoride useful for studying protein folding, protein-protein interactions, and enzyme kinetics. In enzymatic research, it is specifically used to probe the active sites of serine proteases, where it acts as an irreversible inhibitor, binding covalently to the serine residue in the enzyme′s active site. Additionally, Dansyl fluoride is used in the investigation of post-translational modifications of proteins, such as identifying sites of proteolytic cleavage. The compound′s utility extends to the field of analytical chemistry, where it is involved in the development of chromatographic methods for analyzing amines and amino acids.
Dansyl fluoride (CAS 34523-28-9) References
- Identification of the active site of HetR protease and its requirement for heterocyst differentiation in the cyanobacterium Anabaena sp. strain PCC 7120. | Dong, Y., et al. 2000. J Bacteriol. 182: 1575-9. PMID: 10692362
- Heat stable proteinase from Thermomonospora fusca. Characterization as a serine proteinase. | Kristjansson, MM. and Kinsella, JE. 1990. Int J Pept Protein Res. 36: 201-7. PMID: 2132918
- Comparative characterization of the substrate-binding subsites in subtilisins DY and Carlsberg by fluorescence and kinetic studies. | Boteva, R., et al. 1988. J Photochem Photobiol B. 1: 461-8. PMID: 3149293
- Dansyl fluoride, a fluorescent inhibitor for the location of tumour cells in human tissues. | Anees, M. and Benbow, EW. 1996. J Enzyme Inhib. 10: 195-201. PMID: 8835945
- Evidence that HetR protein is an unusual serine-type protease. | Zhou, R., et al. 1998. Proc Natl Acad Sci U S A. 95: 4959-63. PMID: 9560210
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dansyl fluoride, 250 mg | sc-218071 | 250 mg | $214.00 | |||
Dansyl fluoride, 1 g | sc-218071A | 1 g | $367.00 |