Molecular structure of DAF-FM DA (cell permeable), CAS Number: 254109-22-3
DAF-FM DA (cell permeable) (CAS 254109-22-3)
See product citations (7)
Alternate Names:
Diaminofluorescein-FM diacetate; 3-Amino, 4-aminomethyl-2′,7′-difluorofluorescein Diacetate
Application:
DAF-FM DA (cell permeable) is a cell-permeable analog of DAF-FM that acts as a cell permeable NO indicator
CAS Number:
254109-22-3
Purity:
≥98%
Molecular Weight:
496.42
Molecular Formula:
C25H18F2N2O7
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
DAF-FM DA (cell permeable) is an analog of DAF-FM that is cell permeable and can be hydrolyzed to DAF-FM via intracellular esterases. It acts as a nitric oxide (NO) fluorescent indicator. DAF-FM DA (cell permeable) is converted to DAF-FM T when it is exposed simultaneously to both oxygen and NO. It is photostable and stable in pH 5.5 to 12.
DAF-FM DA (cell permeable) (CAS 254109-22-3) References
- Fluorescent Indicators for Imaging Nitric Oxide Production. | Kojima, H., et al. 1999. Angew Chem Int Ed Engl. 38: 3209-3212. PMID: 10556905
- Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry. | Itoh, Y., et al. 2000. Anal Biochem. 287: 203-9. PMID: 11112265
- Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia. | Kojima, H., et al. 2001. J Neurochem. 76: 1404-10. PMID: 11238725
- Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore. | Kojima, H., et al. 2001. Anal Chem. 73: 1967-73. PMID: 11354477
- On the specificity of 4-amino-5-methylamino-2',7'-difluorofluorescein as a probe for nitric oxide. | Balcerczyk, A., et al. 2005. Free Radic Biol Med. 39: 327-35. PMID: 15993331
- Fluorescence monitoring of ATP-stimulated, endothelium-derived nitric oxide production in channels of a poly(dimethylsiloxane)-based microfluidic device. | D'Amico Oblak, T., et al. 2006. Anal Chem. 78: 3193-7. PMID: 16643013
- Real-time measurement of nitric oxide in single mature mouse skeletal muscle fibres during contractions. | Pye, D., et al. 2007. J Physiol. 581: 309-18. PMID: 17331997
- Imaging of nitric oxide in a living vertebrate using a diamino-fluorescein probe. | Lepiller, S., et al. 2007. Free Radic Biol Med. 43: 619-27. PMID: 17640572
- Extracellular ATP induces nitric oxide production in tomato cell suspensions. | Foresi, NP., et al. 2007. Plant Physiol. 145: 589-92. PMID: 17984199
- Detection of nitric oxide (NO) in marine phytoplankters. | Kim, D., et al. 2008. J Biosci Bioeng. 105: 414-7. PMID: 18499060
- Measurement of intracellular nitric oxide (NO) production in shrimp haemocytes by flow cytometry. | Xian, JA., et al. 2013. Fish Shellfish Immunol. 35: 2032-9. PMID: 24513495
- Measurement of nitric oxide in mast cells with the fluorescent indicator DAF-FM diacetate. | St Laurent, CD., et al. 2015. Methods Mol Biol. 1220: 339-45. PMID: 25388261
- Detection of Nitric Oxide from Chickpea Using DAF Fluorescence and Chemiluminescence Methods. | Kumari, A., et al. 2022. Curr Protoc. 2: e420. PMID: 35441832
- Intracellular production of reactive oxygen species and a DAF-FM-related compound in Aspergillus fumigatus in response to antifungal agent exposure. | Oiki, S., et al. 2022. Sci Rep. 12: 13516. PMID: 35933435
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| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAF-FM DA (cell permeable), 1 mg | sc-205940 | 1 mg | $745.00 |