DAF-FM DA (cell permeable) CAS: 254109-22-3
MF: C25H18F2N2O7
MW: 496.42
A cell-permeable analog of DAF-FM that acts as a cell permeable NO indicator.

DAF-FM DA (cell permeable) (CAS 254109-22-3)

DAF-FM DA (cell permeable) | CAS 254109-22-3 is rated 5.0 out of 5 by 1.
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Alternate Names: Diaminofluorescein-FM diacetate; 3-Amino, 4-aminomethyl-2′,7′-difluorofluorescein Diacetate
Application: DAF-FM DA (cell permeable) is a cell-permeable analog of DAF-FM that acts as a cell permeable NO indicator
CAS Number: 254109-22-3
Purity: ≥98%
Molecular Weight: 496.42
Molecular Formula: C25H18F2N2O7
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

DAF-FM DA is an analog of DAF-FM that is cell permeable and can be hydrolyzed to DAF-FM via intracellular esterases. It acts as a nitric oxide (NO) fluorescent indicator. DAF-FM DA is converted to DAF-FM T when it is exposed simultaneously to both oxygen and NO. Photostable and stable in pH 5.5 to 12.


References

1. Itoh, Y., et al., 2000. Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry. Analytical biochemistry. 287(2): 203-9. PMID: 11112265
2. Kojima, H., et al., 2001. Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia. Journal of neurochemistry. 76(5): 1404-10. PMID: 11238725
3. Kojima, H., et al., 2001. Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore. Analytical chemistry. 73(9): 1967-73. PMID: 11354477
4. Lepiller, Sandrine., et al., 2007. Imaging of nitric oxide in a living vertebrate using a diamino-fluorescein probe. Free radical biology & medicine. 43(4): 619-27. PMID: 17640572

Formulation :
~5mM in DMSO
Physical State :
Liquid
Solubility :
Soluble in DMSO.
Storage :
Desiccate at -20° C
Refractive Index :
n20D 1.67 (Predicted)
Fluoresence :
λex 500 nm, λem 515 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
2762646
MDL Number :
MFCD16872050
SMILES :
CC(=O)OC1=C(C=C2C(=C1)OC3=CC(=C(C=C3C24C5=C(C(=C(C=C5)NC)N)C(=O)O4)F)OC(=O)C)F

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Certificate of Analysis

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DAF-FM DA (cell permeable) (CAS 254109-22-3) Product Citations

See how others have used DAF-FM DA (cell permeable) (CAS 254109-22-3). Click on the entry to view the PubMed entry .

Citations 1 to 4 of 4 total

PMID: # 30103245  Guadall, A. et al. 2018. Thromb. Haemost. 118: 1586-1599.

PMID: # 27130531  Fujinaga, H.|Fujinaga, H.|Watanabe, N.|Kato, T.|Tamano, M.|Terao, M.|Takada, S.|Ito, Y.|Umezawa, A.|Kuroda, M.| et al. 2016. Am. J. Physiol. Lung Cell Mol. Physiol. 310: L1143-54.

PMID: # 23658158  Li, LL. et al. 2013. J. Neurosci. 33: 8185-201.

PMID: # 11318983  Cho, JW. et al. 2001. Liver. 21: 137-42.

Citations 1 to 4 of 4 total
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Rated 5 out of 5 by from Yang Yang, T. et al. (PubMed 25724690) used the cell permeable nitric oxide donor DAF-FM DA to demonstrate that inorganic nitrite attenuates NADPH oxidase-derived superoxide generation in activated macrophages via a nitric oxide-dependent mechanism. -SCBT Publication Review
Date published: 2015-05-12
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