DAF-FM DA is an analog of DAF-FM that is cell permeable and can be hydrolyzed to DAF-FM via intracellular esterases. It acts as a nitric oxide (NO) fluorescent indicator. DAF-FM DA is converted to DAF-FM T when it is exposed simultaneously to both oxygen and NO. Photostable and stable in pH 5.5 to 12.
1. Itoh, Y., et al., 2000. Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry. Analytical biochemistry. 287(2): 203-9. PMID: 11112265
2. Kojima, H., et al., 2001. Visualization of oxygen-concentration-dependent production of nitric oxide in rat hippocampal slices during aglycemia. Journal of neurochemistry. 76(5): 1404-10. PMID: 11238725
3. Kojima, H., et al., 2001. Bioimaging of nitric oxide with fluorescent indicators based on the rhodamine chromophore. Analytical chemistry. 73(9): 1967-73. PMID: 11354477
4. Lepiller, Sandrine., et al., 2007. Imaging of nitric oxide in a living vertebrate using a diamino-fluorescein probe. Free radical biology & medicine. 43(4): 619-27. PMID: 17640572
See how others have used DAF-FM DA (cell permeable) (CAS 254109-22-3). Click on the entry to view the PubMed entry .
PMID: # 30103245 Guadall, A. et al. 2018. Thromb. Haemost. 118: 1586-1599.
PMID: # 27130531 Fujinaga, H.|Fujinaga, H.|Watanabe, N.|Kato, T.|Tamano, M.|Terao, M.|Takada, S.|Ito, Y.|Umezawa, A.|Kuroda, M.| et al. 2016. Am. J. Physiol. Lung Cell Mol. Physiol. 310: L1143-54.
PMID: # 23658158 Li, LL. et al. 2013. J. Neurosci. 33: 8185-201.
PMID: # 11318983 Cho, JW. et al. 2001. Liver. 21: 137-42.
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