Date published: 2026-4-24

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DAF-FM (CAS 254109-20-1)

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Alternate Names:
4-Amino-5-methylamino- 2′,7′-difluorofluorescein; Diaminofluorescein-FM
Application:
DAF-FM is a photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM
CAS Number:
254109-20-1
Purity:
≥98%
Molecular Weight:
412.34
Molecular Formula:
C21H14F2N2O5
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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DAF-FM is a photo-stable nitric oxide (NO) fluorescent indicator with a detection limit of ~3 nM. DAF-FM reacts with NO in the presence of oxygen, resulting in a triazolo-fluorescein analog (DAF-FM T) which exhibits about 160-fold greater fluorescence quantum efficiency.


DAF-FM (CAS 254109-20-1) References

  1. Determination and bioimaging method for nitric oxide in biological specimens by diaminofluorescein fluorometry.  |  Itoh, Y., et al. 2000. Anal Biochem. 287: 203-9. PMID: 11112265
  2. Real-time detection of nitric oxide in cultured rat aorta endothelial cells induced by shear stress.  |  Ye, JF., et al. 2003. Sheng Wu Hua Xue Yu Sheng Wu Wu Li Xue Bao (Shanghai). 35: 296-300. PMID: 12621557
  3. Nitric oxide does not trigger early programmed cell death events but may contribute to cell-to-cell signaling governing progression of the Arabidopsis hypersensitive response.  |  Zhang, C., et al. 2003. Mol Plant Microbe Interact. 16: 962-72. PMID: 14601664
  4. On the specificity of 4-amino-5-methylamino-2',7'-difluorofluorescein as a probe for nitric oxide.  |  Balcerczyk, A., et al. 2005. Free Radic Biol Med. 39: 327-35. PMID: 15993331
  5. The novel red-fluorescent probe DAR-4M measures reactive nitrogen species rather than NO.  |  Lacza, Z., et al. 2005. J Pharmacol Toxicol Methods. 52: 335-40. PMID: 16054847
  6. DAF-FM (4-amino-5-methylamino-2',7'-difluorofluorescein) diacetate detects impairment of agonist-stimulated nitric oxide synthesis by elevated glucose in human vascular endothelial cells: reversal by vitamin C and L-sepiapterin.  |  Sheng, JZ., et al. 2005. J Pharmacol Exp Ther. 315: 931-40. PMID: 16093274
  7. Pulmonary alveolar epithelial uptake of S-nitrosothiols is regulated by L-type amino acid transporter.  |  Granillo, OM., et al. 2008. Am J Physiol Lung Cell Mol Physiol. 295: L38-43. PMID: 18441097
  8. Measuring NO production by plant tissues and suspension cultured cells.  |  Vitecek, J., et al. 2008. Mol Plant. 1: 270-84. PMID: 19825539
  9. Role of superoxide-nitric oxide interactions in the accelerated age-related loss of muscle mass in mice lacking Cu,Zn superoxide dismutase.  |  Sakellariou, GK., et al. 2011. Aging Cell. 10: 749-60. PMID: 21443684
  10. Ryanodine receptor leak mediated by caspase-8 activation leads to left ventricular injury after myocardial ischemia-reperfusion.  |  Fauconnier, J., et al. 2011. Proc Natl Acad Sci U S A. 108: 13258-63. PMID: 21788490
  11. Antitumor immunologically modified carbon nanotubes for photothermal therapy.  |  Zhou, F., et al. 2012. Biomaterials. 33: 3235-42. PMID: 22296829
  12. Simultaneous determination of reactive oxygen and nitrogen species in mitochondrial compartments of apoptotic HepG2 cells and PC12 cells based on microchip electrophoresis-laser-induced fluorescence.  |  Chen, Z., et al. 2012. Anal Chem. 84: 4687-94. PMID: 22551384
  13. Measurement of intracellular nitric oxide (NO) production in shrimp haemocytes by flow cytometry.  |  Xian, JA., et al. 2013. Fish Shellfish Immunol. 35: 2032-9. PMID: 24513495
  14. The Role of Quinine-Responsive Taste Receptor Family 2 in Airway Immune Defense and Chronic Rhinosinusitis.  |  Workman, AD., et al. 2018. Front Immunol. 9: 624. PMID: 29643854
  15. Nitric oxide homeostasis is maintained during acute in vitro hypoxia and following reoxygenation in naked mole-rat but not mouse cortical neurons.  |  Wang, TH., et al. 2020. Comp Biochem Physiol A Mol Integr Physiol. 250: 110792. PMID: 32805413

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

DAF-FM, 1 mg

sc-396104
1 mg
$785.00