Date published: 2026-4-25

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DAF-2T (CAS 208850-35-5)

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Alternate Names:
Diaminofluoresceine-2 Triazole
Application:
DAF-2T is a DAF-2 reference material that is a product of DAF-2 and NO reaction
CAS Number:
208850-35-5
Molecular Weight:
373.32
Molecular Formula:
C20H11N3O5
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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DAF-2T is a highly useful fluorescent dye that finds application across numerous scientific domains owing to its remarkable light absorption and emission properties. Derived from fluorescein, DAF-2T has been extensively employed in various research fields such as biochemistry, cell biology, and pharmacology. With an absorption wavelength of 545 nm and an emission wavelength of 565 nm, this dye serves as useful for detecting and visualizing proteins, lipids, and nucleic acids. By harnessing the distinct spectral characteristics of DAF-2T scientists can effectively explore and analyze these biomolecules in diverse experimental contexts.


DAF-2T (CAS 208850-35-5) References

  1. Nitrosative stress by cyclosporin A in the endothelium: studies with the NO-sensitive probe diaminofluorescein-2/diacetate using flow cytometry.  |  Navarro-Antolin, J. and Lamas, S. 2001. Nephrol Dial Transplant. 16 Suppl 1: 6-9. PMID: 11369812
  2. Altered production of nitric oxide and reactive oxygen species in rat nodose ganglion neurons during acute hypoxia.  |  Yamamoto, Y., et al. 2003. Brain Res. 961: 1-9. PMID: 12535770
  3. Thiocyanate cannot inhibit the formation of reactive nitrogen species in the human oral cavity in the presence of high concentrations of nitrite: detection of reactive nitrogen species with 4,5-diaminofluorescein.  |  Takahama, U., et al. 2006. Chem Res Toxicol. 19: 1066-73. PMID: 16918246
  4. Effects of hyperoxia and nitric oxide on endogenous nitric oxide production in polymorphonuclear leukocytes.  |  Hoehn, T., et al. 2008. Neonatology. 94: 132-7. PMID: 18332642
  5. Intracellular nitric oxide assessment in whole blood leukocytes by flow cytometry: optimization and applicability to monitor patients with chronic graft nephropathy.  |  Schachnik, NC., et al. 2009. J Immunol Methods. 343: 103-11. PMID: 19374002
  6. Development of an analytical method for nitric oxide radical determination in natural waters.  |  Olasehinde, EF., et al. 2009. Anal Chem. 81: 6843-50. PMID: 19634896
  7. PRODUCTION OF NITRIC OXIDE WITHIN THE APLYSIA CALIFORNICA NERVOUS SYSTEM.  |  Ye, X., et al. 2010. ACS Chem Neurosci. 1: 182-193. PMID: 20532188
  8. Monitoring of intracellular nitric oxide in leishmaniasis: its applicability in patients with visceral leishmaniasis.  |  Sarkar, A., et al. 2011. Cytometry A. 79: 35-45. PMID: 21182181
  9. Endogenous presynaptic nitric oxide supports an anterograde signaling in the central nervous system.  |  Fernández-Alvarez, A., et al. 2011. J Neurochem. 118: 546-57. PMID: 21644995
  10. Pitfalls and limitations in using 4,5-diaminofluorescein for evaluating the influence of polyphenols on nitric oxide release from endothelial cells.  |  Uhlenhut, K. and Högger, P. Free Radic Biol Med. 52: 2266-75. PMID: 22565032
  11. DAF-fluorescence without NO: elicitor treated tobacco cells produce fluorescing DAF-derivatives not related to DAF-2 triazol.  |  Rümer, S., et al. 2012. Nitric Oxide. 27: 123-35. PMID: 22683597
  12. Methods to Detect Nitric Oxide in Plants: Are DAFs Really Measuring NO?  |  Ruemer, S., et al. 2016. Methods Mol Biol. 1424: 57-68. PMID: 27094411
  13. Phenotypic and functional features of innate and adaptive immunity as putative biomarkers for clinical status and leprosy reactions.  |  Carvalho, JC., et al. 2018. Microb Pathog. 125: 230-239. PMID: 30195647

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

DAF-2T, 1 mg

sc-294266
1 mg
$411.00