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DAF-2 DA (cell permeable) (CAS 205391-02-2)

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Alternate Names:
5,6-Diaminofluorescein diacetate
Application:
DAF-2 DA (cell permeable) is suitable for the fluorimetric detection of nitric oxide
CAS Number:
205391-02-2
Molecular Weight:
446.41
Molecular Formula:
C24H18N2O7
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.

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DAF-2 DA (cell permeable) plays a functional role in studying cellular processes and pathways. When introduced into cells, DAF-2 DA (cell permeable) is rapidly hydrolyzed by intracellular esterases to form DAF-2, which further oxidizes by reactive oxygen and nitrogen species to produce a fluorescent compound. This fluorescent compound serves as an indicator of cellular nitric oxide (NO) levels, facilitating the monitoring of NO in various cellular processes. The fluorescence intensity of DAF-2 provides insights into the regulation of NO production and its impact on cellular signaling, inflammation, and oxidative stress. The mechanism of action of DAF-2 DA (cell permeable) involves specific interactions with NO and its derivatives, allowing for the investigation of NO′s role in cellular physiology and pathology. DAF-2 DA (cell permeable) probe enables the visualization and quantification of NO levels in real-time, illuminating the complex interplay between NO and various cellular components. DAF-2 DA (cell permeable) is utilized in the study of NO in cellular processes and pathways, enhancing understanding of the complex mechanisms underlying cellular physiology.


DAF-2 DA (cell permeable) (CAS 205391-02-2) References

  1. Anti-NO action of carvedilol in cell-free system and in vascular endothelial cells.  |  Yoshioka, T., et al. 2000. Br J Pharmacol. 129: 1530-5. PMID: 10742311
  2. Hypotonic stress-induced NO production in endothelium depends on endogenous ATP.  |  Kimura, C., et al. 2000. Biochem Biophys Res Commun. 274: 736-40. PMID: 10924346
  3. Impairment of endothelial nitric oxide production by acute glucose overload.  |  Kimura, C., et al. 2001. Am J Physiol Endocrinol Metab. 280: E171-8. PMID: 11120671
  4. Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging.  |  Broillet, M., et al. 2001. FEBS Lett. 491: 227-32. PMID: 11240132
  5. Formation of peroxynitrite in vascular endothelial cells exposed to cyclosporine A.  |  Navarro-Antolín, J., et al. 2001. FASEB J. 15: 1291-3. PMID: 11344117
  6. Reliable in vitro measurement of nitric oxide released from endothelial cells using low concentrations of the fluorescent probe 4,5-diaminofluorescein.  |  Leikert, JF., et al. 2001. FEBS Lett. 506: 131-4. PMID: 11591386
  7. Nitric oxide ameliorates hydrophobic bile acid-induced apoptosis in isolated rat hepatocytes by non-mitochondrial pathways.  |  Gumpricht, E., et al. 2002. J Biol Chem. 277: 25823-30. PMID: 12006578
  8. BMP controls nitric oxide-mediated regulation of cell numbers in the developing neural tube.  |  Traister, A., et al. 2004. Cell Death Differ. 11: 832-41. PMID: 15060574
  9. Direct intracellular nitric oxide detection in isolated adult cardiomyocytes: flow cytometric analysis using the fluorescent probe, diaminofluorescein.  |  Strijdom, H., et al. 2004. J Mol Cell Cardiol. 37: 897-902. PMID: 15380680
  10. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore.  |  Kojima, H., et al. 1998. Chem Pharm Bull (Tokyo). 46: 373-5. PMID: 9501473
  11. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins.  |  Nakatsubo, N., et al. 1998. FEBS Lett. 427: 263-6. PMID: 9607324
  12. Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins.  |  Kojima, H., et al. 1998. Anal Chem. 70: 2446-53. PMID: 9666719
  13. Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA.  |  Kojima, H., et al. 1998. Neuroreport. 9: 3345-8. PMID: 9855277

Ordering Information

Product NameCatalog #UNITPriceQtyFAVORITES

DAF-2 DA (cell permeable), 1 mg

sc-221526
1 mg
$428.00