DAF-2 DA (cell permeable) CAS: 205391-02-2
MF: C24H18N2O7
MW: 446.41
Suitable for the fluorimetric detection of nitric oxide.

DAF-2 DA (cell permeable) (CAS 205391-02-2)

DAF-2 DA (cell permeable) | CAS 205391-02-2 is rated 5.0 out of 5 by 1.
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Alternate Names: 5,6-Diaminofluorescein diacetate
Application: DAF-2 DA (cell permeable) is suitable for the fluorimetric detection of nitric oxide
CAS Number: 205391-02-2
Molecular Weight: 446.41
Molecular Formula: C24H18N2O7
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
Submit a review for this product and receive 15 CruzCredits

DAF-2 DA is a cell permeable analog of DAF-2 that is hydrolyzed to DAF-2 by intracellular esterases. It is valuable in fluorescence microscopy for measuring real-time changes in nitric oxide (NO) levels.


References

1. Kojima, H., et al., 1998. Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore. Chemical & pharmaceutical bulletin. 46(2): 373-5. PMID: 9501473
2. Nakatsubo, N., et al., 1998. Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins. FEBS letters. 427(2): 263-6. PMID: 9607324
3. Kojima, H., et al., 1998. Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins. Analytical chemistry. 70(13): 2446-53. PMID: 9666719
4. Kojima, H., et al., 1998. Direct evidence of NO production in rat hippocampus and cortex using a new fluorescent indicator: DAF-2 DA. Neuroreport. 9(15): 3345-8. PMID: 9855277
5. Yoshioka, T., et al., 2000. Anti-NO action of carvedilol in cell-free system and in vascular endothelial cells. British journal of pharmacology. 129(7): 1530-5. PMID: 10742311
6. Kimura, C., et al., 2000. Hypotonic stress-induced NO production in endothelium depends on endogenous ATP. Biochemical and biophysical research communications. 274(3): 736-40. PMID: 10924346
7. Kimura, C., et al., 2001. Impairment of endothelial nitric oxide production by acute glucose overload. American journal of physiology. Endocrinology and metabolism. 280(1): E171-8. PMID: 11120671
8. Broillet, M., et al., 2001. Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging. FEBS letters. 491(3): 227-32. PMID: 11240132
9. Navarro-Antolín, J., et al., 2001. Formation of peroxynitrite in vascular endothelial cells exposed to cyclosporine A. FASEB journal : official publication of the Federation of American Societies for Experimental Biology. 15(7): 1291-3. PMID: 11344117
10. Gumpricht, Eric., et al., 2002. Nitric oxide ameliorates hydrophobic bile acid-induced apoptosis in isolated rat hepatocytes by non-mitochondrial pathways. The Journal of biological chemistry. 277(28): 25823-30. PMID: 12006578
11. Traister, A., et al., 2004. BMP controls nitric oxide-mediated regulation of cell numbers in the developing neural tube. Cell death and differentiation. 11(8): 832-41. PMID: 15060574

Appearance :
Powder
Physical State :
Solid
Solubility :
Soluble in DMSO (>5 mM).
Storage :
Store at -20° C
Boiling Point :
719.03° C at 760 mmHg (Predicted)
Density :
1.54 g/cm3 (Predicted)
Refractive Index :
n20D 1.72 (Predicted)
Fluoresence :
λex 491 nm, λem 513 nm
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
WGK Germany :
3
PubChem CID :
6603693
MDL Number :
MFCD05865101
SMILES :
CC(=O)OC1=CC2=C(C=C1)C3(C4=C(O2)C=C(C=C4)OC(=O)C)C5=CC(=C(C=C5C(=O)O3)N)N

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DAF-2 DA (cell permeable) (CAS 205391-02-2) Product Citations

See how others have used DAF-2 DA (cell permeable) (CAS 205391-02-2). Click on the entry to view the PubMed entry .

Citations 1 to 6 of 6 total

PMID: # 25940071  Baruch, K. et al. 2015. EMBO J. 34: 1816-28.

PMID: # 25019574  Fujita, M. et al. 2014. FEBS Lett. 588: 3240-50.

PMID: # 25310225  Yeon, SI. et al. 2014. PLoS ONE. 9: e110413.

PMID: # 23069711  Hoshi, R.A. et al. 2013. Biomaterials. 34: 30-41.

PMID: # 22750409  Hinits, Y. et al. 2012. Dev. Biol. 369: 199-210.

PMID: # 26202603  Hinits, Y. et al. 2011. Developmental biology. 358: 102-12.

Citations 1 to 6 of 6 total
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Rated 5 out of 5 by from Various publications have cited DAF Various publications have cited DAF-2 DA (cell permeable) for its use in fluorescence microscopy for measuring real-time changes in nitric oxide (NO) levels. -SCBT Publication Review
Date published: 2015-01-27
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