DAF-2 CAS: 205391-01-1
MF: C20H14N2O5
MW: 362.3
A sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro.

DAF-2 (CAS 205391-01-1)

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Alternate Names: 4,5-Diaminofluorescein
Application: DAF-2 is a sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro
CAS Number: 205391-01-1
Molecular Weight: 362.3
Molecular Formula: C20H14N2O5
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data (including water content).
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DAF-2 is a sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. In the presence of oxygen, the relatively non-fluorescent DAF-2 reacts rapidly with NO to yield the highly fluorescent triazolofluorescein compound. DAF-2 can detect NO under neutral conditions (detection limit = 5 nM).


References

1. Berkels, R., et al. 2000. Cell Calcium. 27: 281-286. PMID: 10859594
2. Kojima, H., et al. 1998. Anal. Chem. 70: 2446-2453. PMID: 9666719
3. Kojima, H., et al. 1998. Chem. Pharm. Bull. 46: 373-375. PMID: 9501473
4. Nakatsubo, N., et al. 1998. FEBS Lett. 427: 263-266. PMID: 9607324

Appearance :
Powder
Physical State :
Solid
Storage :
Store at -20° C
Fluoresence :
λex 495 nm, λem 515 nm in DMSO
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
PubChem CID :
10666340
SMILES :
C1=CC2=C(C=C1O)OC3=C(C24C5=CC(=C(C=C5C(=O)O4)N)N)C=CC(=C3)O

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Certificate of Analysis

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