Molecular structure of DAF-2, CAS Number: 205391-01-1
DAF-2 (CAS 205391-01-1)
See product citations (2)
Alternate Names:
4,5-Diaminofluorescein
Application:
DAF-2 is a sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro
CAS Number:
205391-01-1
Molecular Weight:
362.3
Molecular Formula:
C20H14N2O5
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
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SDS & Certificate of Analysis
A sensitive and specific fluorescent indicator for the direct detection of nitric oxide (NO) in vitro. In the presence of oxygen, the relatively non-fluorescent DAF-2 reacts rapidly with NO to yield the highly fluorescent triazolofluorescein compound. Can detect NO under neutral conditions (detection limit = 5 nM). A 5 mM (1 mg in 550 µl) solution in DMSO.
DAF-2 (CAS 205391-01-1) References
- Simultaneous measurement of intracellular Ca(2+) and nitric oxide: a new method. | Berkels, R., et al. 2000. Cell Calcium. 27: 281-6. PMID: 10859594
- Photoactivation and calcium sensitivity of the fluorescent NO indicator 4,5-diaminofluorescein (DAF-2): implications for cellular NO imaging. | Broillet, M., et al. 2001. FEBS Lett. 491: 227-32. PMID: 11240132
- Orthogonality of calcium concentration and ability of 4,5-diaminofluorescein to detect NO. | Suzuki, N., et al. 2002. J Biol Chem. 277: 47-9. PMID: 11641405
- Interfering with nitric oxide measurements. 4,5-diaminofluorescein reacts with dehydroascorbic acid and ascorbic acid. | Zhang, X., et al. 2002. J Biol Chem. 277: 48472-8. PMID: 12370177
- Measurement of nitric oxide by 4,5-diaminofluorescein without interferences. | Ye, X., et al. 2004. Analyst. 129: 1200-5. PMID: 15565218
- A novel method of measuring nitric-oxide-dependent fluorescence using 4,5-diaminofluorescein (DAF-2) in the isolated Langendorff-perfused rabbit heart. | Patel, VH., et al. 2008. Pflugers Arch. 456: 635-45. PMID: 18180949
- Pitfalls and limitations in using 4,5-diaminofluorescein for evaluating the influence of polyphenols on nitric oxide release from endothelial cells. | Uhlenhut, K. and Högger, P. Free Radic Biol Med. 52: 2266-75. PMID: 22565032
- Spontaneous increases in the fluorescence of 4,5-diaminofluorescein and its analogs: their impact on the fluorometry of nitric oxide production in endothelial cells. | Gan, N., et al. 2012. Biol Pharm Bull. 35: 1454-9. PMID: 22975495
- A fluorogenic assay for methylglyoxal. | Shaheen, F., et al. 2014. Biochem Soc Trans. 42: 548-55. PMID: 24646277
- Development of a fluorescent indicator for nitric oxide based on the fluorescein chromophore. | Kojima, H., et al. 1998. Chem Pharm Bull (Tokyo). 46: 373-5. PMID: 9501473
- Direct evidence of nitric oxide production from bovine aortic endothelial cells using new fluorescence indicators: diaminofluoresceins. | Nakatsubo, N., et al. 1998. FEBS Lett. 427: 263-6. PMID: 9607324
- Detection and imaging of nitric oxide with novel fluorescent indicators: diaminofluoresceins. | Kojima, H., et al. 1998. Anal Chem. 70: 2446-53. PMID: 9666719
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAF-2, 1 mg | sc-205910 | 1 mg | $379.00 |