DAD1 Double Nickase Plasmid (h): sc-404172-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- DAD1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- DAD1 Double Nickase Plasmid (h) and DAD1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DAD1. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
DAD1 Double Nickase Plasmid (h) | sc-404172-NIC | 20 µg | $410.00 | |||
DAD1 Double Nickase Plasmid (h2) | sc-404172-NIC-2 | 20 µg | $410.00 |
DAD1 (defender against apoptotic death 1) encodes a conserved subunit of the oligosaccharyltransferase (OST) complex in the endoplasmic reticulum, supporting N-linked glycosylation during co-translational protein processing. By coupling glycan transfer to nascent polypeptides, DAD1 influences protein folding quality control, ER homeostasis, and proteostasis-dependent signaling. Disruption of DAD1 function can sensitize cells to ER stress–associated apoptosis and alter maturation and trafficking of secretory and membrane proteins. These properties make DAD1 a useful node for studying glycoprotein biogenesis, unfolded protein response pathways, and disease-relevant proteostasis mechanisms.
DAD1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the DAD1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within DAD1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt DAD1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of DAD1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.