Dab1 (h): 293T Lysate: sc-112502
- human Dab1 transfected 293T lysate
- 100 µg protein in 200 µl SDS-PAGE buffer; suitable as Western Blotting control for Dab1 antibodies
- Suitable control antibody: Dab1 Antibody (G-5): sc-271136 is recommended as control antibody for monitoring of Dab1 expression knockdown by Western blotting or immunofluorescence
- should be stored at -20° C and repeated freezing and thawing should be minimized
- sample vial should be boiled once prior to use
- non-transfected control 293T lysate (sc-117752)
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The Dab1 (h): 293T Lysate is derived from the HEK293T cell line, a variant of the human embryonic kidney 293 cells that is frequently used in research due to its high transfection efficiency. The lysate contains Disabled-1 (Dab1), an intracellular adaptor protein involved in the Reelin signaling pathway, which plays a critical role in neuronal development and synaptic plasticity. Dab1 functions by interacting with Reelin receptors, leading to the phosphorylation of Dab1 and subsequent activation of downstream signaling cascades such as the PI3K-Akt and Src family kinase pathways. This lysate is valuable for investigating the molecular mechanisms underlying neuronal migration and positioning during brain development. It is used in in vitro assays to study protein-protein interactions, post-translational modifications, and the regulatory mechanisms controlling Dab1 activity. Additionally, the Dab1 (h): 293T Lysate aids in screening for potential modulators of Reelin-Dab1 signaling, facilitating research into the cellular processes governed by this pathway. By utilizing this lysate, researchers can better understand the role of Dab1 in neural circuitry and its impact on synaptic function and plasticity, contributing to the broader field of neurobiology and cell signaling.
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Dab1 (h): 293T Lysate | sc-112502 | 100 µg/200 µl | $236.00 |