D2DR/Dopamine D2 Receptor Antibody (B-10) - Western Blotting - Image 353730
D2DR/Dopamine D2 Receptor Double Nickase Plasmid (h): sc-400235-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- D2DR/Dopamine D2 Receptor Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- D2DR/Dopamine D2 Receptor Double Nickase Plasmid (h) and D2DR/Dopamine D2 Receptor Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting DRD2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: D2DR/Dopamine D2 Receptor Antibody (B-10): sc-5303
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
D2DR/Dopamine D2 Receptor Double Nickase Plasmid (h) | sc-400235-NIC | 20 µg | $410.00 | |||
D2DR/Dopamine D2 Receptor Double Nickase Plasmid (h2) | sc-400235-NIC-2 | 20 µg | $410.00 |