D-type cyclin [HHV8-encoded] Antibody (94B): sc-53271
- D-type cyclin [HHV8-encoded] Antibody (94B) is a rat monoclonal IgG1 D-type cyclin [HHV8-encoded] antibody provided at 200 µg/ml
- raised against recombinant HHV-8-encoded D-type cylin
- D-type cyclin [HHV8-encoded] Antibody (94B) is recommended for detection of HHV8-encoded D-type cylin (vcyc) of HHV-8 origin by WB, IP and IF
- Anti-D-type cyclin [HHV8-encoded] Antibody (94B) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- Contact our Technical Service Department (or your local Distributor) for more information on how to receive a FREE 10 µg sample of D-type cyclin [HHV8-encoded] (94B): sc-53271.
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for D-type cyclin [HHV8-encoded] Antibody (94B). This work is in progress.
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D-type cyclin [HHV8-encoded] Antibody (94B) is a rat monoclonal IgG1 antibody that detects D-type cyclin protein of HHV-8 origin by western blotting (WB), immunoprecipitation (IP), and immunofluorescence (IF). Anti-D-type cyclin [HHV8-encoded] antibody (94B) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. D-type cyclin protein plays a crucial role in regulating the cell cycle, particularly during the G1 phase, where D-type cyclin is essential for the transition to the S phase. This regulation ensures cells only proceed to DNA synthesis when ready, preventing uncontrolled proliferation that can lead to tumorigenesis. The D-type cyclin family, including cyclin D1, has been implicated in various cancers, as D-type cyclin interacts with cyclin-dependent kinases to phosphorylate the retinoblastoma protein, thereby releasing E2F transcription factors that promote the expression of genes necessary for DNA replication. Understanding D-type cyclin function is important for developing targeted therapies in oncology, as D-type cyclin dysregulation is often associated with malignancies.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
D-type cyclin [HHV8-encoded] Antibody (94B) References:
- Cloning of a D-type cyclin from murine erythroleukemia cells. | Kiyokawa, H., et al. 1992. Proc Natl Acad Sci U S A. 89: 2444-7. PMID: 1372445
- Cloning and characterization of human cyclin D3, a cDNA closely related in sequence to the PRAD1/cyclin D1 proto-oncogene. | Motokura, T., et al. 1992. J Biol Chem. 267: 20412-5. PMID: 1383201
- Molecular cloning and chromosomal mapping of CCND genes encoding human D-type cyclins. | Xiong, Y., et al. 1992. Genomics. 13: 575-84. PMID: 1386336
- Growth-regulated expression of D-type cyclin genes in human diploid fibroblasts. | Won, KA., et al. 1992. Proc Natl Acad Sci U S A. 89: 9910-4. PMID: 1409718
- Human D-type cyclin. | Xiong, Y., et al. 1991. Cell. 65: 691-9. PMID: 1827756
- Cell cycle control in eukaryotes: molecular mechanisms of cdc2 activation. | Draetta, G. 1990. Trends Biochem Sci. 15: 378-83. PMID: 2147521
- Cyclin D-CDK4/6 functions in cancer. | Gao, X., et al. 2020. Adv Cancer Res. 148: 147-169. PMID: 32723562
- AMBRA1 regulates cyclin D to guard S-phase entry and genomic integrity. | Maiani, E., et al. 2021. Nature. 592: 799-803. PMID: 33854232
- CRL4AMBRA1 is a master regulator of D-type cyclins. | Simoneschi, D., et al. 2021. Nature. 592: 789-793. PMID: 33854235
- D-Type Cyclins in Development and Disease. | Saleban, M., et al. 2023. Genes (Basel). 14: PMID: 37510349
- CDK-independent role of D-type cyclins in regulating DNA mismatch repair. | Rona, G., et al. 2024. Mol Cell. 84: 1224-1242.e13. PMID: 38458201
- D-type cyclins. | Sherr, CJ. 1995. Trends Biochem Sci. 20: 187-90. PMID: 7610482
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
D-type cyclin [HHV8-encoded] Antibody (94B) | sc-53271 | 200 µg/ml | $316.00 | |||
D-type cyclin [HHV8-encoded] Antibody (94B) AC | sc-53271 AC | 500 µg/ml, 25% agarose | $416.00 | |||
D-type cyclin [HHV8-encoded] Antibody (94B) HRP | sc-53271 HRP | 200 µg/ml | $316.00 | |||
D-type cyclin [HHV8-encoded] Antibody (94B) FITC | sc-53271 FITC | 200 µg/ml | $330.00 | |||
D-type cyclin [HHV8-encoded] Antibody (94B) PE | sc-53271 PE | 200 µg/ml | $343.00 | |||
D-type cyclin [HHV8-encoded] Antibody (94B) Alexa Fluor® 488 | sc-53271 AF488 | 200 µg/ml | $357.00 | |||
D-type cyclin [HHV8-encoded] Antibody (94B) Alexa Fluor® 546 | sc-53271 AF546 | 200 µg/ml | $357.00 | |||
D-type cyclin [HHV8-encoded] Antibody (94B) Alexa Fluor® 594 | sc-53271 AF594 | 200 µg/ml | $357.00 | |||
D-type cyclin [HHV8-encoded] Antibody (94B) Alexa Fluor® 647 | sc-53271 AF647 | 200 µg/ml | $357.00 | |||
D-type cyclin [HHV8-encoded] Antibody (94B) Alexa Fluor® 680 | sc-53271 AF680 | 200 µg/ml | $357.00 | |||
D-type cyclin [HHV8-encoded] Antibody (94B) Alexa Fluor® 790 | sc-53271 AF790 | 200 µg/ml | $357.00 |