D,L-Alanosine-15N2

D,L-Alanosine-15N2, while lacking a specific CAS number in your query, refers to a labeled variant of D,L-alanosine, where the molecule includes two nitrogen atoms that have been isotopically labeled with nitrogen-15 (15N). D,L-Alanosine is a synthetic analog of the amino acid L-alanine. The incorporation of the 15N isotope makes this compound particularly useful in various types of biochemical and biophysical research, especially in studies involving nitrogen metabolism and tracking through enzymatic systems. The primary mechanism of action of D,L-alanosine involves the inhibition of glutamine synthetase, an enzyme critical for the biosynthesis of glutamine from glutamate and ammonia. By inhibiting this enzyme, D,L-Alanosine-15N2 disrupts the ammonia detoxification process and the synthesis of glutamine, which is vital for various metabolic pathways, including the synthesis of nucleotides. In research settings, the labeled nitrogen atoms allow scientists to trace and analyze the pathways of nitrogen incorporation and distribution in biological systems, providing insights into cellular metabolism and the role of nitrogen-containing compounds. This analytical capability is crucial for studies aimed at understanding metabolic fluxes and the impact of metabolic disruptions caused by enzyme inhibitors like D,L-alanosine.