D-erythro-MAPP (CAS 143492-38-0)

D-erythro-MAPP (N-Methyl-2-(1-pyrrolidinyl)-1-pentanamine) is a potent and selective inhibitor of sphingosine kinase, an enzyme responsible for the phosphorylation of sphingosine to sphingosine-1-phosphate (S1P). S1P is a crucial lipid signaling molecule involved in regulating various cellular processes such as proliferation, migration, and survival. By inhibiting sphingosine kinase, D-erythro-MAPP reduces the levels of S1P, thereby modulating signaling pathways dependent on this molecule. In scientific research, D-erythro-MAPP is utilized to explore the role of sphingosine kinase and S1P in cell signaling, particularly in studies related to cancer biology, inflammation, and immune responses. Researchers employ this inhibitor to dissect the contributions of S1P signaling in cellular functions and to understand how alterations in this pathway affect disease progression. Techniques such as mass spectrometry, enzyme activity assays, and cell-based assays are commonly used to assess the impact of D-erythro-MAPP on sphingosine kinase activity and S1P levels. Additionally, studies involving D-erythro-MAPP contribute to the identification of novel targets within the sphingolipid metabolic pathway, enhancing the understanding of lipid signaling networks. Overall, D-erythro-MAPP is a valuable tool in biochemical and cellular research, providing insights into the mechanisms and consequences of sphingosine kinase inhibition.