Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cytokeratin 19 Double Nickase Plasmid (m): sc-421344-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cytokeratin 19 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cytokeratin 19 Double Nickase Plasmid (m) and Cytokeratin 19 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Krt19. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cytokeratin 19 Antibody (A-3): sc-376126
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cytokeratin 19 Double Nickase Plasmid (m) | sc-421344-NIC | 20 µg | $410.00 | |||
Cytokeratin 19 Double Nickase Plasmid (m2) | sc-421344-NIC-2 | 20 µg | $410.00 |
Mouse Krt19 encodes cytokeratin 19 (K19), a type I intermediate filament that forms obligate heteropolymers with type II keratins to support epithelial cytoskeletal architecture and mechanical resilience. K19 contributes to cell shape control, adhesion dynamics, and epithelial differentiation programs, and its filament network interfaces with signaling nodes that regulate stress responses, polarity, and cytoskeleton remodeling. As a marker enriched in simple epithelia and certain progenitor-like epithelial states, K19 is frequently used to stratify epithelial subtypes and to monitor transitions such as remodeling and lineage commitment. Dysregulated K19 expression and filament organization are associated with altered epithelial integrity and are widely studied in contexts of tissue injury, inflammation, and oncogenic transformation in mouse models.
Cytokeratin 19 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Krt19 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Krt19. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Krt19 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Krt19-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.