Date published: 2025-9-5

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Cytokeratin 18 抗体 (RGE53): sc-32329

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说明书
  • Cytokeratin 18抗体(RGE53)是小鼠单克隆IgG1 κ, 在51篇文献中引用,规格为200 µg/ml
  • 与细胞骨架制备结合
  • 抗-Cytokeratin 18 抗体 (RGE53) 推荐用于 WB, IP, IHC(frozen) 和 FCM,检测mouse, rat, human 和bovine 来源的 Cytokeratin 18; 不建议用于paraffin-embedded sections
  • 抗Cytokeratin 18抗体(RGE53)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cytokeratin 18 (RGE53): sc-32329免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRPm-IgGκ BP-HRP是Cytokeratin 18 Antibody (RGE53) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Cytokeratin 18 Antibody (RGE53) 打包提供(请参阅下面的订购信息)。

快捷链接

関連項目

细胞角蛋白18抗体(RGE53)是一种小鼠单克隆IgG1 kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫组织化学(冷冻)和流式细胞术(FCM)等方法检测小鼠、大鼠、人类和牛的细胞角蛋白18。抗细胞角蛋白18抗体(RGE53)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。细胞角蛋白18是上皮细胞细胞骨架的重要组成部分,在维持细胞完整性和提供机械支撑方面发挥着关键作用。细胞角蛋白18主要位于细胞质中,形成中间丝,为上皮组织的结构框架做出贡献。细胞角蛋白18的正确定位对于上皮细胞的正常功能至关重要,有助于抵抗机械应力和促进细胞粘附。细胞角蛋白18的表达或定位变化可作为多种病理状况的重要生物标记,包括癌症,其中腺癌和基底细胞癌的表达通常会升高。抗细胞角蛋白18抗体(RGE53)能够检测多种物种,因此成为研究上皮生物学和疾病的研究人员的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cytokeratin 18 抗体 (RGE53) 参考文献:

  1. 定义人类细胞角蛋白 18 分子表位的新型单克隆抗体。  |  Lauerová, L., et al. 1988. Hybridoma. 7: 495-504. PMID: 2461901
  2. 识别细胞角蛋白 18 号不同表位的单克隆抗体。  |  Vojtĕsek, B., et al. 1989. Folia Biol (Praha). 35: 373-82. PMID: 2483834
  3. 血清细胞角蛋白18作为乳腺外佩吉特病的转移和治疗标志物。  |  Urano-Takaoka, M., et al. 2022. Acta Derm Venereol. 102: adv00636. PMID: 34904690
  4. 先天性阻塞性肾病患儿血清和尿液中的骨膜素和细胞角蛋白-18。  |  Turczyn, A., et al. 2022. Cent Eur J Immunol. 47: 63-72. PMID: 35600153
  5. 肝细胞凋亡片段产物细胞角蛋白-18 M30水平与非酒精性脂肪性肝炎风险诊断:一项国际登记研究。  |  Zhang, H., et al. 2023. Chin Med J (Engl). 136: 341-350. PMID: 36848175
  6. 评估健康人和前列腺癌患者细胞角蛋白 8 和 18 片段的新型肿瘤标记物。  |  Silén, A., et al. 1994. Prostate. 24: 326-32. PMID: 7516071
  7. 用于定量检测健康人和癌症患者血清中细胞角蛋白 8 和 18 片段的新型 IRMA 和 ELISA。  |  Silén, A., et al. 1995. Scand J Clin Lab Invest. 55: 153-61. PMID: 7545308
  8. 正常和(恶性前)头颈部上皮细胞角蛋白的表达:综述。  |  van der Velden, LA., et al. 1993. Head Neck. 15: 133-46. PMID: 7680025
  9. 肺癌患者血清和组织中细胞角蛋白 19(cyfra 21-1)片段的分布。  |  Quillien, V., et al. 1995. Anticancer Res. 15: 2857-63. PMID: 8669879
  10. 通过反义 RNA 使 p53 功能失活可诱导肺癌细胞的侵袭能力并下调细胞角蛋白的合成。  |  Mukhopadhyay, T. and Roth, JA. 1996. Anticancer Res. 16: 1683-9. PMID: 8712687
  11. 肝细胞中细胞角蛋白的表达, 纤维组织和微妙功能。  |  Marceau, N. and Loranger, A. 1995. Biochem Cell Biol. 73: 619-25. PMID: 8714681
  12. 人角蛋白 18 丝氨酸 33 的磷酸化调节与 14-3-3 蛋白的结合。  |  Ku, NO., et al. 1998. EMBO J. 17: 1892-906. PMID: 9524113

订购信息

产品名称产品编号规格价格数量收藏夹

Cytokeratin 18 抗体 (RGE53)

sc-32329
200 µg/ml
$316.00

Cytokeratin 18 (RGE53): m-IgGκ BP-HRP 套装

sc-520849
200 µg Ab, 40 µg BP
$354.00

Cytokeratin 18 (RGE53): m-IgG1 BP-HRP 套装

sc-533472
200 µg Ab; 20 µg BP
$354.00

Cytokeratin 18 抗体 (RGE53) AC

sc-32329 AC
500 µg/ml, 25% agarose
$416.00

Cytokeratin 18 抗体 (RGE53) HRP

sc-32329 HRP
200 µg/ml
$316.00

Cytokeratin 18 抗体 (RGE53) FITC

sc-32329 FITC
200 µg/ml
$330.00

Cytokeratin 18 抗体 (RGE53) PE

sc-32329 PE
200 µg/ml
$343.00

Cytokeratin 18 抗体 (RGE53) Alexa Fluor® 488

sc-32329 AF488
200 µg/ml
$357.00

Cytokeratin 18 抗体 (RGE53) Alexa Fluor® 546

sc-32329 AF546
200 µg/ml
$357.00

Cytokeratin 18 抗体 (RGE53) Alexa Fluor® 594

sc-32329 AF594
200 µg/ml
$357.00

Cytokeratin 18 抗体 (RGE53) Alexa Fluor® 647

sc-32329 AF647
200 µg/ml
$357.00

Cytokeratin 18 抗体 (RGE53) Alexa Fluor® 680

sc-32329 AF680
200 µg/ml
$357.00

Cytokeratin 18 抗体 (RGE53) Alexa Fluor® 790

sc-32329 AF790
200 µg/ml
$357.00

How can Cytokeratin 18 (RGE53): sc-32329 monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jenni
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. Cytokeratin 18 (RGE53): sc-32329 monoclonal antibody is currently available conjugated to phycoerythrin (sc-32329 PE), fluorescein (sc-32329 FITC), Alexa Fluor® 488 (sc-32329 AF488) or Alexa Fluor® 647 (sc-32329 AF647).
Answered by: Technical Support 5
Date published: 2017-01-10
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Rated 2 out of 5 by from Western blotThis antibody had good expression in western blot before, but the new lot didn't work well . (gastric cancer cell line: AGS,SCM-1)
Date published: 2017-11-21
Rated 5 out of 5 by from Hep G2 whole cell lysateStrong band with this antibody using 1:800 for wb in Hep G2 whole cell lysate
Date published: 2017-05-07
Rated 5 out of 5 by from Strong for multiple applications !!Outstanding results in western blotting, IHC and IF studies
Date published: 2017-01-14
Rated 5 out of 5 by from Excellent for IF studiesThe Cytokeratin 18 Antibody (RGE53) is a great antibody for immunofluorescent applications, showing strong and localized staining patterns with multiple publication citations.
Date published: 2016-12-28
Rated 4 out of 5 by from Used this antibody for bovine Immunofluorescenceit is ok for bovine Immunofluorescence in BMEC. Reliable antibody.
Date published: 2016-11-15
Rated 5 out of 5 by from good staining in paraffin embedded samplesgood staining in paraffin embedded samples (mouse uterus)
Date published: 2015-11-16
Rated 5 out of 5 by from Beautiful immunofluorescence staining ofBeautiful immunofluorescence staining of the glandular portion of human cervix. -SCBT QC
Date published: 2015-04-07
Rated 5 out of 5 by from WB: Single clean band in Hep G2 samplesWB: Single clean band in Hep G2 samples. -SCBT QC
Date published: 2015-04-05
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Cytokeratin 18 抗体 (RGE53) is rated 4.6 out of 5 by 11.
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