Cytokeratin 18 Antibody (B23.1.): sc-58727

Cytokeratin 18 Antibody (B23.1) is a mouse monoclonal IgG1 antibody that detects Cytokeratin 18 in human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Cytokeratin 18 is a vital component of the cytoskeleton in epithelial cells, contributing significantly to structural integrity and resilience. Cytokeratin 18 is predominantly located in the cytoplasm, forming a network of intermediate filaments that provide mechanical support and shape to cells. Proper localization of Cytokeratin 18 is crucial for maintaining epithelial tissue architecture, and disruption can lead to various pathological conditions, including cancer. Cytokeratin 18 serves as a biomarker for epithelial-derived tumors, as expression levels can indicate differentiation status of malignant cells. Additionally, Cytokeratin 18 undergoes post-translational modifications, including phosphorylation, which influences protein interactions and overall function within cells. Phosphorylation of specific serine residues, such as Ser 33 and Ser 52, is particularly important for stability and interaction with 14-3-3 proteins, which play a role in regulating keratin organization and distribution. Anti-Cytokeratin 18 antibody (B23.1) enables researchers to gain valuable insights into normal physiology and disease states, making this tool essential for studies in cancer research and tissue biology.

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Cytokeratin 18 Antibody (B23.1.) References:

1. Vimentin, cytokeratin 8 and cytokeratin 18 are not specific markers for M-cells in human palatine tonsils.  |  Koshi, R., et al. 2001. J Anat. 199: 663-74. PMID: 11787820
2. Diagnostic value of cytokeratin-18 as a tumor marker in bladder cancer.  |  Ramazan Sekeroğlu, M., et al. 2002. Clin Biochem. 35: 327-31. PMID: 12135697
3. Cytokeratin 18 fragment levels as a noninvasive biomarker for nonalcoholic steatohepatitis in bariatric surgery patients.  |  Diab, DL., et al. 2008. Clin Gastroenterol Hepatol. 6: 1249-54. PMID: 18995215
4. Proteomic analysis to identify cytokeratin 18 as a novel biomarker of nasopharyngeal carcinoma.  |  Li, XM., et al. 2009. J Cancer Res Clin Oncol. 135: 1763-75. PMID: 19529960
5. Cytokeratin 18 is a specific marker of bovine intestinal M cell.  |  Hondo, T., et al. 2011. Am J Physiol Gastrointest Liver Physiol. 300: G442-53. PMID: 21193527
6. Cytokeratin 18 is not required for morphogenesis of developing prostates but contributes to adult prostate regeneration.  |  Zhang, C., et al. 2013. Biomed Res Int. 2013: 576472. PMID: 24672777
7. Cytokeratin 14 and cytokeratin 18 expressions in reduced enamel epithelium and dentigerous cyst: Possible role in oncofetal transformation and histogenesis- of follicular type of adenomatoid odontogenic tumor.  |  Shruthi, DK., et al. 2014. J Oral Maxillofac Pathol. 18: 365-71. PMID: 25948990
8. Non-Survivor Ischemic Stroke Patients Maintain High Serum Caspase-Cleaved Cytokeratin-18 Levels.  |  Lorente, L., et al. 2020. Brain Sci. 10: PMID: 32120809
9. Accuracy of cytokeratin 18 (M30 and M65) in detecting non-alcoholic steatohepatitis and fibrosis: A systematic review and meta-analysis.  |  Lee, J., et al. 2020. PLoS One. 15: e0238717. PMID: 32915852
10. Diagnostic and prognostic impact of cytokeratin 18 expression in human tumors: a tissue microarray study on 11,952 tumors.  |  Menz, A., et al. 2021. Mol Med. 27: 16. PMID: 33588765

The preferred Cytokeratin 18 antibody is Cytokeratin 18 Antibody (RGE53): sc-32329.