Cytokeratin 18 Antibody (B23.1.): sc-58727

Cytokeratin 18 Antibody (B23.1) is a mouse monoclonal IgG1 antibody that detects Cytokeratin 18 in human samples through western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and flow cytometry (FCM). Cytokeratin 18 is a vital component of the cytoskeleton in epithelial cells, contributing significantly to structural integrity and resilience. Cytokeratin 18 is predominantly located in the cytoplasm, forming a network of intermediate filaments that provide mechanical support and shape to cells. Proper localization of Cytokeratin 18 is crucial for maintaining epithelial tissue architecture, and disruption can lead to various pathological conditions, including cancer. Cytokeratin 18 serves as a biomarker for epithelial-derived tumors, as expression levels can indicate differentiation status of malignant cells. Additionally, Cytokeratin 18 undergoes post-translational modifications, including phosphorylation, which influences protein interactions and overall function within cells. Phosphorylation of specific serine residues, such as Ser 33 and Ser 52, is particularly important for stability and interaction with 14-3-3 proteins, which play a role in regulating keratin organization and distribution. Anti-Cytokeratin 18 antibody (B23.1) enables researchers to gain valuable insights into normal physiology and disease states, making this tool essential for studies in cancer research and tissue biology.

The preferred Cytokeratin 18 antibody is Cytokeratin 18 Antibody (RGE53): sc-32329.