cytochrome c1 Double Nickase Plasmid (h): sc-404958-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cytochrome c1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- cytochrome c1 Double Nickase Plasmid (h) and cytochrome c1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CYC1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: cytochrome c1 Antibody (A-5): sc-514435
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cytochrome c1 Double Nickase Plasmid (h) | sc-404958-NIC | 20 µg | $410.00 | |||
cytochrome c1 Double Nickase Plasmid (h2) | sc-404958-NIC-2 | 20 µg | $410.00 |
Human CYC1 encodes cytochrome c1, an essential subunit of mitochondrial complex III (cytochrome bc1 complex) that transfers electrons from ubiquinol to cytochrome c within the inner mitochondrial membrane. This activity supports oxidative phosphorylation by contributing to proton translocation that helps establish the electrochemical gradient used for ATP synthesis. Perturbation of complex III components can alter mitochondrial redox balance and reactive oxygen species production, linking CYC1 biology to pathways governing cellular energy metabolism and stress responses. Dysregulated mitochondrial respiration and electron transport chain integrity are frequently studied in the context of metabolic remodeling and neurodegeneration-associated mitochondrial dysfunction.
cytochrome c1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CYC1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CYC1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CYC1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CYC1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.