Cysteine Antibody (3A4): sc-69954

Cysteine Antibody (3A4) is a mouse monoclonal IgG2a antibody that detects the Cysteine protein of various species by western blotting (WB), immunoprecipitation (IP), and flow cytometry (FCM). Anti-Cysteine antibody (3A4) is available as a non-conjugated format. Cysteine is a non-essential α-amino acid characterized by its free thiol (-SH) side chain, which plays a crucial role in the structure and function of numerous proteins. This amino acid is found abundantly in dietary sources such as eggs, milk, pork, oats, onions, and garlic, and is recognized for antioxidant properties and ability to bind metal ions, making Cysteine valuable in pharmaceutical, food, and personal care industries. Notably, Cysteine is the most chemically reactive amino acid under physiological conditions, readily oxidizing to form cystine, a dimer linked by a disulfide bond. The significance of Cysteine extends to post-translational modifications, particularly cysteinylation, which involves the addition of a cysteine residue to a peptide. This modification is one of the most prevalent in cellular processes and can serve as a biomarker for various physiological states, including inflammation and oxidative stress, highlighting Cysteine′s importance in maintaining cellular health and responding to environmental changes.

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Cysteine Antibody (3A4) References:

1. Modification of cysteine residues in vitro and in vivo affects the immunogenicity and antigenicity of major histocompatibility complex class I-restricted viral determinants.  |  Chen, W., et al. 1999. J Exp Med. 189: 1757-64. PMID: 10359579
2. Cysteinylation of MHC class II ligands: peptide endocytosis and reduction within APC influences T cell recognition.  |  Haque, MA., et al. 2001. J Immunol. 166: 4543-51. PMID: 11254711
3. PKC isozyme S-cysteinylation by cystine stimulates the pro-apoptotic isozyme PKC delta and inactivates the oncogenic isozyme PKC epsilon.  |  Chu, F., et al. 2003. Carcinogenesis. 24: 317-25. PMID: 12584183
4. Cysteinylation of maternal plasma albumin and its association with intrauterine growth restriction.  |  Bar-Or, D., et al. 2005. Prenat Diagn. 25: 245-9. PMID: 15791656
5. Iron-sulfur protein biogenesis in eukaryotes: components and mechanisms.  |  Lill, R. and Mühlenhoff, U. 2006. Annu Rev Cell Dev Biol. 22: 457-86. PMID: 16824008
6. S-cysteinylation is a general mechanism for thiol protection of Bacillus subtilis proteins after oxidative stress.  |  Hochgräfe, F., et al. 2007. J Biol Chem. 282: 25981-5. PMID: 17611193
7. Removal of cysteinylation from an unpaired sulfhydryl in the variable region of a recombinant monoclonal IgG1 antibody improves homogeneity, stability, and biological activity.  |  Banks, DD., et al. 2008. J Pharm Sci. 97: 775-90. PMID: 17786988
8. Structure-based Approaches Targeting Parasite Cysteine Proteases.  |  Vieira, RP., et al. 2019. Curr Med Chem. 26: 4435-4453. PMID: 28799498
9. Pharmacologic role of cysteine in ameliorating or exacerbating mineral toxicities.  |  Baker, DH. and Czarnecki-Maulden, GL. 1987. J Nutr. 117: 1003-10. PMID: 3298579
10. Cysteine and related aminothiols in cardiovascular disease, obesity and insulin resistance.  |  Pavão, ML., et al. 2022. Adv Clin Chem. 109: 75-127. PMID: 35953129
11. Diversity and roles of cysteine desulfurases in photosynthetic organisms.  |  Caubrière, D., et al. 2023. J Exp Bot. 74: 3345-3360. PMID: 36861318
12. Protective action of sulfur compounds against aldehyde toxicants of cigarette smoke.  |  Sprince, H. 1985. Eur J Respir Dis Suppl. 139: 102-12. PMID: 3862602

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-Cysteine Antibody (3A4) has 0 citations in a variety of scientific publications.

Primary antibodies for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

Cysteine Antibody (3A4) Specifications

Alternate Product Name: Cysteine

Product Clone Name: 3A4

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids:

Epitope Target: Cysteine

Epitope Species: mouse

Product Reactivity/Detection: Cysteine

Species Reactivity/Detection: N/A

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, FCM

Vialing Information: Each vial contains 100 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for Cysteine Antibody (3A4): N/A