cystatin B Antibody (F-5): sc-166561
- cystatin B Antibody (F-5) is a mouse monoclonal IgG1 κ cystatin B antibody, cited in 3 publications, provided at 200 µg/ml
- raised against amino acids 51-98 mapping at the C-terminus of cystatin B of human origin
- cystatin B Antibody (F-5) is recommended for detection of cystatin B of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA
- Anti-cystatin B Antibody (F-5) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for cystatin B Antibody (F-5) for WB and IHC(P) applications. These reagents are now offered in bundles with cystatin B Antibody (F-5) (see ordering information below).
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Cystatin B Antibody (F-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects cystatin B protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). Anti-cystatin B antibody (F-5) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. Cystatin B, also known as CSTB, plays a crucial role as a thiol protease inhibitor, forming complexes with proteases such as papain and cathepsins B, H, and L, which are essential for protein degradation and turnover within cells. Cystatin B is primarily located in the cytoplasm and is significantly expressed in the heart, liver, and kidney, where cystatin B serves to protect against the leakage of intracellular proteases from lysosomes, thereby maintaining cellular integrity and function. Dysregulation of cystatin B has been implicated in various pathological conditions, including neurodegenerative diseases, highlighting cystatin B′s importance in cellular homeostasis and potential as a therapeutic target.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
cystatin B Antibody (F-5) References:
- Expression of human cystatin A by keratinocytes is positively regulated via the Ras/MEKK1/MKK7/JNK signal transduction pathway but negatively regulated via the Ras/Raf-1/MEK1/ERK pathway. | Takahashi, H., et al. 2001. J Biol Chem. 276: 36632-8. PMID: 11451947
- Different propensity to form amyloid fibrils by two homologous proteins-Human stefins A and B: searching for an explanation. | Jenko, S., et al. 2004. Proteins. 55: 417-25. PMID: 15048832
- Cloning a synthetic gene for human stefin B and its expression in E. coli. | Jerala, R., et al. 1988. FEBS Lett. 239: 41-4. PMID: 3053245
- Amino acid sequence of the intracellular cysteine proteinase inhibitor cystatin B from human liver. | Ritonja, A., et al. 1985. Biochem Biophys Res Commun. 131: 1187-92. PMID: 3902020
- Mutations in the gene encoding cystatin B in progressive myoclonus epilepsy (EPM1). | Pennacchio, LA., et al. 1996. Science. 271: 1731-4. PMID: 8596935
- Structure and transcriptional regulation of the human cystatin A gene. The 12-O-tetradecanoylphorbol-13-acetate (TPA) responsive element-2 site (-272 to -278) on cystatin A gene is critical for TPA-dependent regulation. | Takahashi, H., et al. 1998. J Biol Chem. 273: 17375-80. PMID: 9651321
- Cysteine proteinases and their endogenous inhibitors: target proteins for prognosis, diagnosis and therapy in cancer (review). | Kos, J. and Lah, TT. 1998. Oncol Rep. 5: 1349-61. PMID: 9769367
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cystatin B Antibody (F-5) | sc-166561 | 200 µg/ml | $322.00 | |||
cystatin B Antibody (F-5): m-IgG Fc BP-HRP Bundle | sc-528995 | 200 µg Ab; 10 µg BP | $361.00 | |||
cystatin B Antibody (F-5): m-IgGκ BP-HRP Bundle | sc-521657 | 200 µg Ab, 40 µg BP | $361.00 | |||
cystatin B Antibody (F-5): m-IgG1 BP-HRP Bundle | sc-543256 | 200 µg Ab; 20 µg BP | $361.00 | |||
cystatin B Antibody (F-5) AC | sc-166561 AC | 500 µg/ml, 25% agarose | $424.00 | |||
cystatin B Antibody (F-5) HRP | sc-166561 HRP | 200 µg/ml | $322.00 | |||
cystatin B Antibody (F-5) FITC | sc-166561 FITC | 200 µg/ml | $336.00 | |||
cystatin B Antibody (F-5) PE | sc-166561 PE | 200 µg/ml | $349.00 | |||
cystatin B Antibody (F-5) Alexa Fluor® 488 | sc-166561 AF488 | 200 µg/ml | $364.00 | |||
cystatin Antibody (F-5) Alexa Fluor® 546 | sc-166561 AF546 | 200 µg/ml | $364.00 | |||
cystatin B Antibody (F-5) Alexa Fluor® 594 | sc-166561 AF594 | 200 µg/ml | $364.00 | |||
cystatin B Antibody (F-5) Alexa Fluor® 647 | sc-166561 AF647 | 200 µg/ml | $364.00 | |||
cystatin B Antibody (F-5) Alexa Fluor® 680 | sc-166561 AF680 | 200 µg/ml | $364.00 | |||
cystatin B Antibody (F-5) Alexa Fluor® 790 | sc-166561 AF790 | 200 µg/ml | $364.00 |