CYP27A1 Antibody (D-2): sc-514693
- CYP27A1 Antibody (D-2) is a mouse monoclonal IgM κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 454-476 near the C-terminus of CYP27A1 of human origin
- recommended for detection of CYP27A1 of human origin by WB, IP, IF and ELISA
- At present, we have not yet completed the identification of the preferred secondary detection reagent(s) for CYP27A1 Antibody (D-2). This work is in progress.
CYP27A1 Antibody (D-2) is a mouse monoclonal IgM antibody that detects CYP27A1 in human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CYP27A1, also known as cholesterol 27-hydroxylase, plays a crucial role in the metabolism of cholesterol, converting it into bile acids and other metabolites, which are essential for maintaining cholesterol homeostasis and facilitating lipid digestion. The enzyme is primarily localized in the mitochondria of liver and kidney cells, where CYP27A1 catalyzes the hydroxylation of vitamin D3, a process vital for the activation of vitamin D and regulation of calcium homeostasis in the body. Dysregulation of CYP27A1 has been linked to various metabolic disorders, including atherosclerosis and vitamin D deficiency, highlighting CYP27A1′s importance in both cholesterol metabolism and overall health. Anti-CYP27A1 antibody (D-2) is an invaluable tool for researchers studying these pathways and their implications in human disease.
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CYP27A1 Antibody (D-2) References:
- Glucocorticoid receptor-mediated upregulation of human CYP27A1, a potential anti-atherogenic enzyme. | Tang, W., et al. 2008. Biochim Biophys Acta. 1781: 718-23. PMID: 18817892
- Role of CYP27A1 in progesterone metabolism in vitro and in vivo. | Escher, G., et al. 2009. Am J Physiol Endocrinol Metab. 297: E949-55. PMID: 19671838
- β-Cryptoxanthin uptake in THP-1 macrophages upregulates the CYP27A1 signaling pathway. | Fu, H., et al. 2014. Mol Nutr Food Res. 58: 425-36. PMID: 24123714
- Hematopoietic overexpression of Cyp27a1 reduces hepatic inflammation independently of 27-hydroxycholesterol levels in Ldlr(-/-) mice. | Hendrikx, T., et al. 2015. J Hepatol. 62: 430-6. PMID: 25281859
- Mast cells express CYP27A1 and CYP27B1 in epithelial skin cancers and psoriasis. | Kaukinen, A., et al. 2015. Eur J Dermatol. 25: 548-55. PMID: 26463745
- Hepatic caveolin-1 is enhanced in Cyp27a1/ApoE double knockout mice. | Zurkinden, L., et al. 2016. FEBS Open Bio. 6: 1025-1035. PMID: 28149711
- Impact of 27-hydroxylase (CYP27A1) and 27-hydroxycholesterol in breast cancer. | Kimbung, S., et al. 2017. Endocr Relat Cancer. 24: 339-349. PMID: 28442559
- CYP27A1 expression is associated with risk of late lethal estrogen receptor-positive breast cancer in postmenopausal patients. | Kimbung, S., et al. 2020. Breast Cancer Res. 22: 123. PMID: 33176848
- High CYP27A1 expression is a biomarker of favorable prognosis in premenopausal patients with estrogen receptor positive primary breast cancer. | Inasu, M., et al. 2021. NPJ Breast Cancer. 7: 127. PMID: 34556659
- Methyl-CpG-binding protein 2 regulates CYP27A1-induced myometrial contraction during preterm labor. | Peng, T., et al. 2024. Mol Hum Reprod. 30: PMID: 38704863
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CYP27A1 Antibody (D-2) | sc-514693 | 200 µg/ml | $322.00 | |||
CYP27A1 (D-2) Neutralizing Peptide | sc-514693 P | 100 µg/0.5 ml | $69.00 |