CYP1A1 (h): 293T Lysate: sc-114027

CYP1A1 (h): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line genetically modified to express the SV40 large T antigen, which enhances its transfection efficiency and protein production capabilities. This lysate is enriched with human Cytochrome P450 1A1 (CYP1A1), an enzyme that plays a crucial role in the metabolism of xenobiotics, including the bioactivation and detoxification of various environmental pollutants, drugs, and endogenous compounds. CYP1A1 is involved in the oxidative metabolism of polycyclic aromatic hydrocarbons (PAHs) and other aromatic hydrocarbons, converting them into more water-soluble forms for excretion. In research, CYP1A1 (h): 293T Lysate is utilized to study the enzyme's metabolic pathways and regulatory mechanisms. Researchers employ this lysate in Western blotting to detect and quantify CYP1A1 expression under different experimental conditions, providing insights into its induction by environmental contaminants and regulatory factors. The lysate is also used in enzymatic activity assays to measure the metabolism of specific substrates, helping to explain the catalytic properties and substrate specificity of CYP1A1. Additionally, CYP1A1 (h): 293T Lysate is valuable in co-immunoprecipitation assays to identify protein-protein interactions involving CYP1A1, which aids in understanding its integration within larger metabolic and signaling networks. Functional assays using this lysate can assess the impact of various inducers and inhibitors on CYP1A1 activity, contributing to a deeper understanding of its role in chemical metabolism and detoxification.