Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cyclophilin F Double Nickase Plasmid (h): sc-402902-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cyclophilin F Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cyclophilin F Double Nickase Plasmid (h) and Cyclophilin F Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting PPIF. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cyclophilin F Antibody (G-9): sc-376061
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cyclophilin F Double Nickase Plasmid (h) | sc-402902-NIC | 20 µg | $410.00 | |||
Cyclophilin F Double Nickase Plasmid (h2) | sc-402902-NIC-2 | 20 µg | $410.00 |
PPIF encodes cyclophilin F, a mitochondrial peptidyl-prolyl cis-trans isomerase that supports protein folding and proteostasis within the matrix and inner membrane environment. Cyclophilin F contributes to regulation of mitochondrial permeability transition and oxidative stress responses, linking it to control of mitochondrial membrane potential, reactive oxygen species handling, and apoptosis-related signaling. Through these processes, PPIF intersects with energy metabolism and cell survival pathways that are frequently perturbed in neurodegeneration, ischemia-reperfusion injury models, and cancer-associated mitochondrial remodeling. Altered PPIF activity has therefore been used to probe how mitochondrial stress integrates with inflammatory and cell death programs in human cell systems.
Cyclophilin F Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the PPIF locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within PPIF. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt PPIF function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of PPIF-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.