Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
cyclin T1 Double Nickase Plasmid (h): sc-400623-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cyclin T1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- cyclin T1 Double Nickase Plasmid (h) and cyclin T1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CCNT1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: cyclin T1 Antibody (E-3): sc-271348
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin T1 Double Nickase Plasmid (h) | sc-400623-NIC | 20 µg | $410.00 | |||
cyclin T1 Double Nickase Plasmid (h2) | sc-400623-NIC-2 | 20 µg | $410.00 |
CCNT1 encodes cyclin T1, a regulatory subunit of the P-TEFb complex that partners with CDK9 to phosphorylate the RNA polymerase II C-terminal domain and promote transcriptional elongation. Through control of pause release, cyclin T1 influences stimulus-responsive gene expression programs, cell-cycle progression, and differentiation-associated transcriptional networks. CCNT1-dependent P-TEFb activity also interfaces with chromatin regulation and RNA processing, linking transcription dynamics to broader gene regulatory circuitry. Dysregulated cyclin T1/CDK9 signaling has been implicated in oncogenic transcriptional states and other disorders characterized by altered transcriptional control, supporting its use in mechanistic studies of transcription-dependent phenotypes.
cyclin T1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CCNT1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CCNT1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CCNT1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CCNT1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.