cyclin G2 Double Nickase Plasmid (h): sc-403620-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cyclin G2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- cyclin G2 Double Nickase Plasmid (h) and cyclin G2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CCNG2. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: cyclin G2 Antibody (1F9-C11): sc-293302
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cyclin G2 Double Nickase Plasmid (h) | sc-403620-NIC | 20 µg | $410.00 | |||
cyclin G2 Double Nickase Plasmid (h2) | sc-403620-NIC-2 | 20 µg | $410.00 |
CCNG2 encodes cyclin G2, an atypical cyclin that is frequently induced by growth-inhibitory cues and contributes to negative regulation of cell cycle progression, particularly around the G1/S transition. Cyclin G2 interfaces with stress-responsive signaling and can modulate checkpoint control and cellular proliferation through interactions that influence phosphatase activity and phosphorylation-dependent pathways. Altered CCNG2 expression has been associated with dysregulated proliferation and differentiation programs, making it relevant for studies of tumor biology, tissue homeostasis, and context-dependent growth suppression. Its regulatory role also links it to cellular responses such as senescence and adaptation to genotoxic or metabolic stress.
cyclin G2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CCNG2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CCNG2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CCNG2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CCNG2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.