Cyanine 3-dUTP
Alternate Names:
Cyanine 3-dUTP is also known as Cyanine 3-deoxyuridine-5′-triphosphate.
Application:
Cyanine 3-dUTP is used to replace TTP in reactions as a substrate for E. coli DNA polymerase, T4 and Taq DNA polymerases, reverse transcriptase and terminal transferase.
Purity:
≥93%
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.
* Refer to Certificate of Analysis for lot specific data.
QUICK LINKS
Ordering Information
Description
Technical Information
Safety Information
SDS & Certificate of Analysis
Cyanine 3-dUTP can be used to replace TTP in reactions in which it serves as a substrate for E. coli DNA polymerase (holoenzyme and Klenow fragment), T4 and Taq DNA polymerases, reverse transcriptase (from AMV and M-MuLV) and terminal transferase. Cyanine 3-dUTP is also useful because it can be used to prepare fluorescently labeled probes by a variety of methods including nick translation, cDNA labeling and 3′-end labeling. These Cyanine 3-dUTP probes can then be used for the identification of specific sequences by iin situ hybridization procedures on fixed cells and tissues by direct fluorescence detection. Additionally, Cyanine 3-dUTP can be used for multicolor fluorescence labeling. Avoid Freeze/Thaw cycles when using Cyanine 3-dUTP.
Cyanine 3-dUTP References
- Distinct roles of activating transcription factor 6 (ATF6) and double-stranded RNA-activated protein kinase-like endoplasmic reticulum kinase (PERK) in transcription during the mammalian unfolded protein response. | Okada, T., et al. 2002. Biochem J. 366: 585-94. PMID: 12014989
- CD24 is expressed specifically in the nucleus pulposus of intervertebral discs. | Fujita, N., et al. 2005. Biochem Biophys Res Commun. 338: 1890-6. PMID: 16288985
- Adenovirus-mediated PEDF expression inhibits prostate cancer cell growth and results in augmented expression of PAI-2. | Guan, M., et al. 2007. Cancer Biol Ther. 6: 419-25. PMID: 17471021
- Growth inhibitory and apoptosis inducing effect of Perilla frutescens extract on human hepatoma HepG2 cells. | Lin, CS., et al. 2007. J Ethnopharmacol. 112: 557-67. PMID: 17574356
- Two-dimensional differential gel electrophoresis/analysis of diethylnitrosamine induced rat hepatocellular carcinoma. | Qi, Y., et al. 2008. Int J Cancer. 122: 2682-8. PMID: 18351647
- Case report on the analysis of single nucleotide polymorphisms of the serotonergic pathway and array-based comparative genomic hybridisation of a suicide victim. | Videtic, A., et al. 2008. Psychiatr Danub. 20: 245-8. PMID: 18587299
- Coupling global methylation and gene expression profiles reveal key pathophysiological events in liver injury induced by a methyl-deficient diet. | Tryndyak, VP., et al. 2011. Mol Nutr Food Res. 55: 411-8. PMID: 20938992
- Refractory anemia with ring sideroblasts associated with marked thrombocytosis harboring cytogenetic abnormality dup(2)(p15p22) treated with decitabine. | Kim, J., et al. 2012. Leuk Lymphoma. 53: 2287-9. PMID: 22468921
- A genome-wide survey of copy number variation regions in various chicken breeds by array comparative genomic hybridization method. | Wang, Y., et al. 2012. Anim Genet. 43: 282-9. PMID: 22486499
- Identification of putative target genes for amplification within 11q13.2 and 3q27.1 in esophageal squamous cell carcinoma. | Shi, ZZ., et al. 2014. Clin Transl Oncol. 16: 606-15. PMID: 24203761
- Analysis of Copy-Number Alterations in Single Cells Using Microarray-Based Comparative Genomic Hybridization (aCGH). | Möhlendick, B. and Stoecklein, NH. 2014. Curr Protoc Cell Biol. 65: 22.19.1-23. PMID: 25447076
- Transport of Nucleoside Triphosphates into Cells by Artificial Molecular Transporters. | Zawada, Z., et al. 2018. Angew Chem Int Ed Engl. 57: 9891-9895. PMID: 29578619
- Detection of apoptosis and DNA replication by differential labeling of DNA strand breaks with fluorochromes of different color. | Li, X., et al. 1996. Exp Cell Res. 222: 28-37. PMID: 8549670
- Cross-species colour segmenting: a novel tool in human karyotype analysis. | Müller, S., et al. 1998. Cytometry. 33: 445-52. PMID: 9845439
Substrate of:
Fluorogenic.Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cyanine 3-dUTP, 25 nmol | sc-472858 | 25 nmol | $265.00 |