Cyanine 3 Bihexanoic Acid Dye, Potassium Salt (CAS 762260-71-9)

Cyanine 3 Bihexanoic Acid Dye, Potassium Salt, with the CAS number 762260-71-9, is a fluorescent dye widely utilized in the field of biochemical and biophysical research for its superior fluorescent properties. This chemical belongs to the cyanine dye family, known for their ability to absorb and emit light at various wavelengths. Specifically, Cyanine 3 dye is characterized by its absorption peak typically around 550 nm and an emission peak near 570 nm, making it visible in the orange to red portion of the spectrum. The "bihexanoic acid" component of the dye refers to the presence of hexanoic acid groups that increase the dye′s solubility and provide reactive sites for conjugation with biomolecules. This facilitates the dye′s use in labeling proteins, nucleic acids, and other biological targets for fluorescence microscopy, flow cytometry, and other fluorescence-based detection methods. The mechanism of action involves the covalent attachment of the dye to biomolecules via its reactive groups, which upon excitation by appropriate wavelengths of light, emit a bright, stable fluorescence. This feature allows researchers to track and analyze the distribution, localization, and dynamics of labeled molecules within cells or in other experimental setups. Cyanine 3 Bihexanoic Acid Dye is particularly valued in research for its relatively high quantum yield and its ability to be excited by common laser lines, which makes it compatible with many fluorescence detection systems. Its use in diverse applications, such as in the study of cellular processes, protein interactions, and nucleic acid analysis, underscores its versatility and effectiveness as a tool in advanced imaging techniques.