CXCR-4 Double Nickase Plasmid (h): sc-400254-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CXCR-4 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CXCR-4 Double Nickase Plasmid (h) and CXCR-4 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CXCR4. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CXCR-4 Antibody (4G10): sc-53534
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CXCR-4 Double Nickase Plasmid (h) | sc-400254-NIC | 20 µg | $410.00 | |||
CXCR-4 Double Nickase Plasmid (h2) | sc-400254-NIC-2 | 20 µg | $410.00 |
CXCR4 encodes the chemokine receptor CXCR-4, a seven-transmembrane GPCR that binds CXCL12/SDF-1 to regulate chemotaxis, cell adhesion, and survival signaling. CXCR-4 activity couples to G-protein and β-arrestin pathways, engaging downstream cascades such as PI3K/AKT, MAPK/ERK, and PLC-mediated calcium flux to coordinate cytoskeletal remodeling and directional migration. This axis is central to leukocyte trafficking and hematopoietic cell retention within bone marrow niches, and it is frequently interrogated in studies of immune cell dynamics and tumor cell dissemination. Dysregulation of CXCR4 signaling and expression is associated with altered inflammatory responses and cancer-associated invasion and metastasis phenotypes, making it a common target for mechanistic pathway analysis.
CXCR-4 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CXCR4 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CXCR4. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CXCR4 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CXCR4-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.