CUL-4 Antibody (H-11): sc-377188. Western blot analysis of CUL-4 expression in PC-12 (A), HeLa (B), NIH/3T3 (C), HL-60 (D) and Jurkat (E) whole cell lysates.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CUL-4 Antibody (H-11): sc-377188. Western blot analysis of CUL-4 expression in PC-12 (A), HeLa (B), NIH/3T3 (C), HL-60 (D) and Jurkat (E) whole cell lysates.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
CUL-4A CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CUL-4A genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines