Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CTP CRISPR/Cas9 KO Plasmid (h): sc-417472
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CTP CRISPR/Cas9 Knockout (KO) Plasmid (h) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the CTP genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CTP Antibody (C-12): sc-518244
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CTP CRISPR/Cas9 KO Plasmid (h) | sc-417472 | 20 µg | $397.00 |
Overview
SLC25A1 encodes the mitochondrial citrate transporter protein (CTP), an inner membrane carrier that exchanges citrate/isocitrate for malate and links mitochondrial metabolism to cytosolic biosynthetic programs. By exporting citrate for ATP-citrate lyase–dependent acetyl-CoA production, CTP supports de novo lipid synthesis, protein acetylation, and epigenetic regulation, while also influencing redox balance through downstream NADPH-generating pathways. SLC25A1 activity integrates with central carbon metabolism, including the TCA cycle, glycolysis, and anaplerotic flux, shaping cellular responses to nutrient availability and mitochondrial stress. Dysregulation of citrate transport has been associated with altered metabolic remodeling observed in cancer, neurodevelopmental disorders, and inflammatory signaling contexts, making SLC25A1 a useful node for mechanistic studies of mitochondrial–cytosolic crosstalk.
CTP CRISPR/Cas9 KO Plasmid (h) is a pool of plasmids designed for targeted disruption of the SLC25A1 gene in human cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the SLC25A1 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the SLC25A1 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish CTP protein expression.
This CRISPR knockout system enables efficient generation of SLC25A1-deficient cell models for investigation of CTP signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting SLC25A1 exon(s) critical for CTP function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple SLC25A1 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by CTP CRISPR/Cas9 KO Plasmid (h) and CTP CRISPR/Cas9 KO Plasmid (h2) target distinct sites within the SLC25A1 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by CTP HDR Plasmid (h) and CTP HDR Plasmid (h2) contain a puromycin resistance cassette and an RFP reporter flanked by SLC25A1 homology arms to support homology-directed repair at defined SLC25A1 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.