Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CTH Double Nickase Plasmid (h): sc-401134-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CTH Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CTH Double Nickase Plasmid (h) and CTH Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CTH. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CTH Antibody (F-1): sc-374249
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CTH Double Nickase Plasmid (h) | sc-401134-NIC | 20 µg | $410.00 | |||
CTH Double Nickase Plasmid (h2) | sc-401134-NIC-2 | 20 µg | $410.00 |
Human CTH encodes cystathionine gamma-lyase, a pyridoxal phosphate–dependent enzyme in the transsulfuration pathway that converts cystathionine to cysteine, alpha-ketobutyrate, and ammonia, linking methionine metabolism to cellular cysteine availability. CTH also contributes to endogenous hydrogen sulfide (H2S) production, influencing redox homeostasis, mitochondrial bioenergetics, and inflammatory signaling. Through its role in cysteine and glutathione metabolism, CTH impacts oxidative stress responses and ferroptosis susceptibility across multiple cell types. Altered CTH expression or activity has been associated with cardiometabolic dysfunction, cancer cell metabolic reprogramming, and neuroinflammation-related phenotypes in experimental models.
CTH Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CTH locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CTH. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CTH function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CTH-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.