Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CtBP2 Double Nickase Plasmid (m): sc-419868-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CtBP2 Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CtBP2 Double Nickase Plasmid (m) and CtBP2 Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Ctbp2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CtBP2 Double Nickase Plasmid (m) | sc-419868-NIC | 20 µg | $410.00 | |||
CtBP2 Double Nickase Plasmid (m2) | sc-419868-NIC-2 | 20 µg | $410.00 |
Mouse Ctbp2 encodes the C-terminal binding protein 2 (CtBP2), a NADH-sensitive transcriptional coregulator that links cellular metabolic state to chromatin remodeling and gene expression. CtBP2 associates with DNA-binding transcription factors and corepressor complexes to modulate histone deacetylation and other epigenetic mechanisms controlling differentiation, cell-cycle programs, and stress-responsive transcription. Through regulation of pathways such as Wnt/β-catenin, TGF-β, and Notch-associated transcriptional outputs, CtBP2 influences epithelial–mesenchymal transition, neuronal development, and synaptic gene networks. Dysregulated CTBP2 activity and altered corepressor balance have been connected to disease-relevant phenotypes including tumor progression, neurodevelopmental defects, and metabolic adaptation in proliferative cells.
CtBP2 Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Ctbp2 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Ctbp2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Ctbp2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Ctbp2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.