Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
CtBP2 Double Nickase Plasmid (h): sc-401865-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CtBP2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CtBP2 Double Nickase Plasmid (h) and CtBP2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CTBP2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CtBP2 Double Nickase Plasmid (h) | sc-401865-NIC | 20 µg | $410.00 | |||
CtBP2 Double Nickase Plasmid (h2) | sc-401865-NIC-2 | 20 µg | $410.00 |
Human CTBP2 encodes C-terminal binding protein 2 (CtBP2), a NADH-sensitive transcriptional coregulator that functions primarily as a corepressor by recruiting chromatin-modifying complexes, including HDACs and other epigenetic regulators, to sequence-specific transcription factors. CtBP2 influences gene programs controlling proliferation, lineage specification, epithelial–mesenchymal transition, and apoptosis, linking cellular metabolic state to transcriptional output. Through these activities, CtBP2 modulates pathways such as Wnt/β-catenin, TGF-β, and hypoxia-associated transcriptional networks, and helps shape chromatin accessibility and enhancer activity. Dysregulated CTBP2 expression or function has been associated with altered differentiation and tumor-associated transcriptional states, making it a useful target for mechanistic studies of transcriptional repression and metabolic control of gene regulation.
CtBP2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CTBP2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CTBP2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CTBP2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CTBP2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.