CSN1 Double Nickase Plasmid (h): sc-404934-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CSN1 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CSN1 Double Nickase Plasmid (h) and CSN1 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting GPS1. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: CSN1 Antibody (E-4): sc-514086
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CSN1 Double Nickase Plasmid (h) | sc-404934-NIC | 20 µg | $410.00 | |||
CSN1 Double Nickase Plasmid (h2) | sc-404934-NIC-2 | 20 µg | $410.00 |
Human GPS1 encodes COP9 signalosome subunit 1 (CSN1), a core component of the COP9 signalosome that regulates cullin-RING ubiquitin ligases by controlling cullin neddylation and coordinating ubiquitin-dependent protein turnover. Through this activity, CSN1 influences cell-cycle progression, DNA damage responses, and stress signaling, intersecting with pathways such as MAPK and NF-κB that shape transcriptional programs and proteostasis. Perturbation of COP9 signalosome function has been linked to altered stability of key regulators including cyclins and transcription factors, contributing to dysregulated proliferation and genome maintenance. GPS1/CSN1 is therefore of interest for mechanistic studies in cancer biology, inflammation-related signaling, and broader investigations of ubiquitin–proteasome pathway control.
CSN1 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the GPS1 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within GPS1. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt GPS1 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of GPS1-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.