CSN1 Antibody (E-4): sc-514086
- CSN1 Antibody (E-4) is a mouse monoclonal IgG1 κ CSN1 antibody provided at 200 µg/ml
- specific for an epitope mapping between amino acids 391-413 near the C-terminus of CSN1 of human origin
- CSN1 Antibody (E-4) is recommended for detection of CSN1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CSN1 Antibody (E-4) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP is the preferred secondary detection reagent for CSN1 Antibody (E-4) for WB applications. This reagent is now offered in a bundle with CSN1 Antibody (E-4) (see ordering information below).
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CSN1 Antibody (E-4) is a mouse monoclonal IgG1 kappa light chain antibody that detects CSN1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). CSN1 (E-4) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CSN1, a crucial component of the COP9 signalosome complex, plays a significant role in regulating the ubiquitin conjugation pathway, which is essential for protein degradation and cellular homeostasis. This complex, composed of several proteins including CSN2, CSN3, and CSN5, is involved in various developmental and cellular processes, such as the deneddylation of SCF-type E3 ligase complexes, which ultimately reduces their ubiquitin ligase activity. CSN1 is implicated in the phosphorylation of key regulatory proteins like p53 and c-Jun, influencing cell cycle progression and apoptosis. CSN1′s ability to modulate these pathways highlights its importance in maintaining cellular function and responding to stress, making CSN1 a valuable target for research in cancer and developmental biology.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CSN1 Antibody (E-4) References:
- The subunit 1 of the COP9 signalosome suppresses gene expression through its N-terminal domain and incorporates into the complex through the PCI domain. | Tsuge, T., et al. 2001. J Mol Biol. 305: 1-9. PMID: 11114242
- COP9 signalosome-specific phosphorylation targets p53 to degradation by the ubiquitin system. | Bech-Otschir, D., et al. 2001. EMBO J. 20: 1630-9. PMID: 11285227
- Promotion of NEDD-CUL1 conjugate cleavage by COP9 signalosome. | Lyapina, S., et al. 2001. Science. 292: 1382-5. PMID: 11337588
- Deletion mutants in COP9/signalosome subunits in fission yeast Schizosaccharomyces pombe display distinct phenotypes. | Mundt, KE., et al. 2002. Mol Biol Cell. 13: 493-502. PMID: 11854407
- Protein kinase CK2 and protein kinase D are associated with the COP9 signalosome. | Uhle, S., et al. 2003. EMBO J. 22: 1302-12. PMID: 12628923
- The ubiquitin ligase activity in the DDB2 and CSA complexes is differentially regulated by the COP9 signalosome in response to DNA damage. | Groisman, R., et al. 2003. Cell. 113: 357-67. PMID: 12732143
- The COP9 signalosome: mediating between kinase signaling and protein degradation. | Harari-Steinberg, O. and Chamovitz, DA. 2004. Curr Protein Pept Sci. 5: 185-9. PMID: 15180523
- Hepatopoietin interacts directly with COP9 signalosome and regulates AP-1 activity. | Wang, Y., et al. 2004. FEBS Lett. 572: 85-91. PMID: 15304329
- Intracellular IL-1 receptor antagonist type 1 inhibits IL-1-induced cytokine production in keratinocytes through binding to the third component of the COP9 signalosome. | Banda, NK., et al. 2005. J Immunol. 174: 3608-16. PMID: 15749898
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CSN1 Antibody (E-4) | sc-514086 | 200 µg/ml | $322.00 | |||
CSN1 Antibody (E-4): m-IgG1 BP-HRP Bundle | sc-531772 | 200 µg Ab; 20 µg BP | $361.00 | |||
CSN1 Antibody (E-4) AC | sc-514086 AC | 500 µg/ml, 25% agarose | $424.00 | |||
CSN1 Antibody (E-4) HRP | sc-514086 HRP | 200 µg/ml | $322.00 | |||
CSN1 Antibody (E-4) FITC | sc-514086 FITC | 200 µg/ml | $336.00 | |||
CSN1 Antibody (E-4) PE | sc-514086 PE | 200 µg/ml | $349.00 | |||
CSN1 Antibody (E-4) Alexa Fluor® 488 | sc-514086 AF488 | 200 µg/ml | $364.00 | |||
CSN1 Antibody (E-4) Alexa Fluor® 546 | sc-514086 AF546 | 200 µg/ml | $364.00 | |||
CSN1 Antibody (E-4) Alexa Fluor® 594 | sc-514086 AF594 | 200 µg/ml | $364.00 | |||
CSN1 Antibody (E-4) Alexa Fluor® 647 | sc-514086 AF647 | 200 µg/ml | $364.00 | |||
CSN1 Antibody (E-4) Alexa Fluor® 680 | sc-514086 AF680 | 200 µg/ml | $364.00 | |||
CSN1 Antibody (E-4) Alexa Fluor® 790 | sc-514086 AF790 | 200 µg/ml | $364.00 | |||
CSN1 (E-4) Neutralizing Peptide | sc-514086 P | 100 µg/0.5 ml | $69.00 |