cryptdin 3 CRISPR/Cas9 KO Plasmid (m): sc-419984
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- cryptdin 3 CRISPR/Cas9 Knockout (KO) Plasmid (m) is a pool of plasmids, each encoding Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed for maximum knockout efficiency using sequences derived from the GeCKO v2 library
- gRNA sequences direct Cas9 to induce site-specific double-strand breaks (DSBs) in the cryptdin 3 genomic locus, resulting in gene knockout through non-homologous end joining (NHEJ)
- The puromycin resistance and RFP genes are flanked by LoxP sites, enabling removal of selection markers via Cre recombinase (Cre Vector: sc-418923) after establishing stable knockout cell lines
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
cryptdin 3 CRISPR/Cas9 KO Plasmid (m) | sc-419984 | 20 µg | $397.00 |
Overview
Defa3 encodes cryptdin 3, a Paneth cell–derived α-defensin that contributes to innate immune defense in the mouse small intestine by directly targeting microbial membranes and shaping the composition of the gut microbiota. As a component of antimicrobial peptide biology, cryptdin 3 participates in epithelial barrier maintenance and mucosal immune homeostasis alongside pattern-recognition and inflammatory signaling pathways. Altered Paneth cell defensin output has been linked to dysbiosis and intestinal inflammation, making Defa3 relevant for mechanistic studies of host–microbe interactions. Defa3 perturbation is also useful for examining how antimicrobial peptides influence bacterial colonization resistance and epithelial stress responses.
cryptdin 3 CRISPR/Cas9 KO Plasmid (m) is a pool of plasmids designed for targeted disruption of the Defa3 gene in mouse cell lines. Each plasmid co-expresses a unique single guide RNA (sgRNA) targeting a distinct site within the Defa3 together with the Streptococcus pyogenes Cas9 nuclease. The plasmids also encode GFP, allowing fluorescent identification and enrichment of successfully transfected cells by fluorescence microscopy or flow cytometry.
The multi-guide design increases the likelihood of generating insertions or deletions (indels) that disrupt the Defa3 open reading frame following Cas9-mediated double-strand break formation. DNA breaks introduced by the CRISPR/Cas9 system are repaired through endogenous non-homologous end joining (NHEJ) pathways, frequently resulting in frameshift mutations that abolish cryptdin 3 protein expression.
This CRISPR knockout system enables efficient generation of Defa3-deficient cell models for investigation of cryptdin 3 signaling, functional genomics studies, cancer biology research, and evaluation of therapeutic responses in human cell lines.
Key Features
- sgRNAs targeting Defa3 exon(s) critical for cryptdin 3 function
- Co-expression of SpCas9 and sgRNA from a single plasmid for simplified delivery
- GFP reporter for identification of transfected cells
- Pool of plasmids targeting multiple Defa3 genomic sites to improve knockout efficiency
- Compatible with delivery by transfection
Design Variants
CRISPRs +/- HDRs
- gRNAs encoded by cryptdin 3 CRISPR/Cas9 KO Plasmid (m) and cryptdin 3 CRISPR/Cas9 KO Plasmid (m2) target distinct sites within the Defa3 locus. One or both targeting designs may be available. See Related Products for availability.
- HDR donor constructs encoded by cryptdin 3 HDR Plasmid (m) and cryptdin 3 HDR Plasmid (m2) contain a puromycin resistance cassette and an RFP reporter flanked by Defa3 homology arms to support homology-directed repair at defined Defa3 target sites corresponding to the CRISPR/Cas9 KO designs. HDR donor availability may vary. See Related Products for availability.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.