CRY1 Antibody (W-L5): sc-101006

CRY1 Antibody (W-L5) is a mouse monoclonal IgG1 kappa light chain antibody that detects CRY1 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CRY1 Antibody (W-L5) is available in a non-conjugated form, specifically designed for researchers seeking to study cryptochrome-1′s role in circadian rhythms. CRY1 functions as a light-independent inhibitor of the circadian clock, interacting with key components such as Clock and BMAL1 to regulate gene expression involved in the light/dark cycle. Located primarily in the nucleus, CRY1 plays a vital role in the negative feedback loop that stabilizes circadian rhythms by forming heterodimers with other proteins like Per1 and Per2, which in turn inhibit Clock-BMAL1 dimer activity. This interaction is essential for proper functioning of the suprachiasmatic nucleus, the brain′s master clock, ensuring that physiological processes such as hormonal rhythms, sleep cycles, and feeding behaviors are synchronized with environmental light cues. Understanding CRY1′s dynamics and interactions is fundamental for elucidating the molecular mechanisms underlying circadian regulation and its implications for health and disease.

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CRY1 Antibody (W-L5) References:

1. Differential regulation of mammalian period genes and circadian rhythmicity by cryptochromes 1 and 2.  |  Vitaterna, MH., et al. 1999. Proc Natl Acad Sci U S A. 96: 12114-9. PMID: 10518585
2. Light-independent role of CRY1 and CRY2 in the mammalian circadian clock.  |  Griffin, EA., et al. 1999. Science. 286: 768-71. PMID: 10531061
3. Mutation of the Human Circadian Clock Gene CRY1 in Familial Delayed Sleep Phase Disorder.  |  Patke, A., et al. 2017. Cell. 169: 203-215.e13. PMID: 28388406
4. CRY1-CBS binding regulates circadian clock function and metabolism.  |  Cal-Kayitmazbatir, S., et al. 2021. FEBS J. 288: 614-639. PMID: 32383312
5. Hepatic GSK3β-Dependent CRY1 Degradation Contributes to Diabetic Hyperglycemia.  |  Kim, YY., et al. 2022. Diabetes. 71: 1373-1387. PMID: 35476750
6. The circadian clock CRY1 regulates pluripotent stem cell identity and somatic cell reprogramming.  |  Sato, S., et al. 2023. Cell Rep. 42: 112590. PMID: 37261952
7. A 24-hour circadian clock is found in the mammalian retina.  |  Morell, V. 1996. Science. 272: 349. PMID: 8602520
8. A differential response of two putative mammalian circadian regulators, mper1 and mper2, to light.  |  Albrecht, U., et al. 1997. Cell. 91: 1055-64. PMID: 9428527
9. Mammalian circadian autoregulatory loop: a timeless ortholog and mPer1 interact and negatively regulate CLOCK-BMAL1-induced transcription.  |  Sangoram, AM., et al. 1998. Neuron. 21: 1101-13. PMID: 9856465
10. Molecular analysis of mammalian timeless.  |  Zylka, MJ., et al. 1998. Neuron. 21: 1115-22. PMID: 9856466
11. Molecular bases for circadian clocks.  |  Dunlap, JC. 1999. Cell. 96: 271-90. PMID: 9988221
12. A molecular mechanism regulating rhythmic output from the suprachiasmatic circadian clock.  |  Jin, X., et al. 1999. Cell. 96: 57-68. PMID: 9989497

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CRY1 Antibody (W-L5) has 5 citations in a variety of scientific publications.

Primary antibodies like Anti-CRY1 Antibody (W-L5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CRY1 Antibody (W-L5) Specifications

Alternate Product Name: Cryptochrome-1

Product Clone Name: W-L5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-587

Epitope Target: CRY1

Epitope Species: human

Product Reactivity/Detection: CRY1

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 100 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 5 citations in various scientific publications

Available Conjugates/Forms for CRY1 Antibody (W-L5): N/A

The preferred CRY1 antibody is CRY1 Antibody (H-12): sc-393466.