CRY1 Antibody (H-12): sc-393466
- CRY1 Antibody (H-12) is a mouse monoclonal IgG1 κ CRY1 antibody, cited in 4 publications, provided at 200 µg/ml
- raised against amino acids 503-586 mapping at the C-terminus of CRY1 of human origin
- CRY1 Antibody (H-12) is recommended for detection of CRY1 of mouse, rat and human origin by WB, IP, IF and ELISA
- Anti-CRY1 Antibody (H-12) is available conjugated to agarose for IP; HRP for WB, IHC(P) and ELISA; and to either phycoerythrin or FITC for IF, IHC(P) and FCM
- also available conjugated to Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 or Alexa Fluor® 647 for WB (RGB), IF, IHC(P) and FCM, and for use with RGB fluorescent imaging systems, such as iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- also available conjugated to Alexa Fluor® 680 or Alexa Fluor® 790 for WB (NIR), IF and FCM; for use with Near-Infrared (NIR) detection systems, such as LI-COR®Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure and other comparable systems
- m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for CRY1 Antibody (H-12) for WB applications. These reagents are now offered in bundles with CRY1 Antibody (H-12) (see ordering information below).
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CRY1 Antibody (H-12) is a mouse monoclonal IgG1 kappa light chain antibody that detects CRY1 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). anti-CRY1 antibody (H-12) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CRY1 plays a crucial role in regulating circadian rhythms, acting as a light-independent inhibitor of the circadian clock. CRY1 is primarily located in the nucleus, where CRY1 interacts with key transcription factors such as Clock and BMAL1 to modulate their activity. This nuclear localization is vital because CRY1 directly influences the expression of genes involved in the circadian cycle, thereby maintaining the synchronization of biological processes with environmental light cues. By forming complexes with other circadian proteins, CRY1 helps establish a feedback loop essential for proper functioning of the circadian clock, ensuring physiological processes such as sleep-wake cycles and hormonal rhythms are appropriately timed.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
CRY1 Antibody (H-12) References:
- SREBP1c-CRY1 signalling represses hepatic glucose production by promoting FOXO1 degradation during refeeding. | Jang, H., et al. 2016. Nat Commun. 7: 12180. PMID: 27412556
- CRY1/2 Selectively Repress PPARδ and Limit Exercise Capacity. | Jordan, SD., et al. 2017. Cell Metab. 26: 243-255.e6. PMID: 28683290
- JMJD5 links CRY1 function and proteasomal degradation. | Saran, AR., et al. 2018. PLoS Biol. 16: e2006145. PMID: 30500822
- Human CRY1 variants associate with attention deficit/hyperactivity disorder. | Onat, OE., et al. 2020. J Clin Invest. 130: 3885-3900. PMID: 32538895
- The circadian cryptochrome, CRY1, is a pro-tumorigenic factor that rhythmically modulates DNA repair. | Shafi, AA., et al. 2021. Nat Commun. 12: 401. PMID: 33452241
- Photopharmacological Manipulation of Mammalian CRY1 for Regulation of the Circadian Clock. | Kolarski, D., et al. 2021. J Am Chem Soc. 143: 2078-2087. PMID: 33464888
- Differential photoregulation of the nuclear and cytoplasmic CRY1 in Arabidopsis. | Liu, S., et al. 2022. New Phytol. 234: 1332-1346. PMID: 35094400
- Resistance of Spodoptera frugiperda to Cry1, Cry2, and Vip3Aa Proteins in Bt Corn and Cotton in the Americas: Implications for the Rest of the World. | Yang, F., et al. 2022. J Econ Entomol. 115: 1752-1760. PMID: 36515105
- Cry1 expression during postnatal development is critical for the establishment of normal circadian period. | Schirmer, AE., et al. 2023. Front Neurosci. 17: 1166137. PMID: 37389366
- Blue light receptor CRY1 regulates HSFA1d nuclear localization to promote plant thermotolerance. | Gao, J., et al. 2023. Cell Rep. 42: 113117. PMID: 37703177
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CRY1 Antibody (H-12) | sc-393466 | 200 µg/ml | $316.00 | |||
CRY1 Antibody (H-12): m-IgGκ BP-HRP Bundle | sc-523957 | 200 µg Ab, 40 µg BP | $354.00 | |||
CRY1 Antibody (H-12): m-IgG1 BP-HRP Bundle | sc-533629 | 200 µg Ab; 20 µg BP | $354.00 | |||
CRY1 Antibody (H-12) AC | sc-393466 AC | 500 µg/ml, 25% agarose | $416.00 | |||
CRY1 Antibody (H-12) HRP | sc-393466 HRP | 200 µg/ml | $316.00 | |||
CRY1 Antibody (H-12) FITC | sc-393466 FITC | 200 µg/ml | $330.00 | |||
CRY1 Antibody (H-12) PE | sc-393466 PE | 200 µg/ml | $343.00 | |||
CRY1 Antibody (H-12) Alexa Fluor® 488 | sc-393466 AF488 | 200 µg/ml | $357.00 | |||
CRY1 Antibody (H-12) Alexa Fluor® 546 | sc-393466 AF546 | 200 µg/ml | $357.00 | |||
CRY1 Antibody (H-12) Alexa Fluor® 594 | sc-393466 AF594 | 200 µg/ml | $357.00 | |||
CRY1 Antibody (H-12) Alexa Fluor® 647 | sc-393466 AF647 | 200 µg/ml | $357.00 | |||
CRY1 Antibody (H-12) Alexa Fluor® 680 | sc-393466 AF680 | 200 µg/ml | $357.00 | |||
CRY1 Antibody (H-12) Alexa Fluor® 790 | sc-393466 AF790 | 200 µg/ml | $357.00 |