Cruz Marker™ MW Tag-Alexa Fluor® 647: sc-516791

Cruz Marker MW Tag-Alexa Fluor 647 is a sophisticated molecular weight marker used predominantly in biochemical research for the precise analysis of proteins via SDS-PAGE and western blotting techniques. This marker combines protein standards with Alexa Fluor 647, a fluorophore known for its intense far-red fluorescence and exceptional photostability, facilitating the accurate visualization of protein migration. The core mechanism involves the proteins, tagged with Alexa Fluor 647, migrating through a gel according to their molecular weights, with their respective positions illuminated by the dye's fluorescence under specific lighting conditions. This direct visualization allows researchers to accurately determine the molecular weight of proteins in their samples by comparing them against the known standards provided in the marker. The far-red emission spectrum of Alexa Fluor 647 is particularly beneficial as it reduces background fluorescence, thereby enhancing the clarity and reliability of data, which is crucial for detailed protein analysis. Employed extensively in proteomics, this marker is instrumental in evaluating protein purity, detecting post-translational modifications, and assessing protein-protein interactions, thus providing a critical tool for advancing our understanding of protein dynamics within various biological contexts.