CRSP150 Antibody (2214C1a): sc-81236

CRSP150 Antibody (2214C1a) is a mouse monoclonal IgG1 antibody that detects CRSP150 protein of mouse, rat, and human origin by western blotting (WB) and immunoprecipitation (IP). Anti-CRSP150 antibody (2214C1a) is available as the non-conjugated form. CRSP150 plays a crucial role in transcription regulation within mammalian cells, functioning as part of the CRSP (cofactor required for Sp1 activation) complex, which mediates signals between transcriptional activators and RNA polymerase. CRSP150 is a key component of a high molecular weight coactivating complex ubiquitously expressed across various tissues, highlighting CRSP150′s importance in gene expression regulation. The CRSP complex, including CRSP150, comprises nine distinct subunits and shares structural similarities with yeast transcriptional mediator proteins, indicating a conserved mechanism of transcriptional regulation across species. CRSP150 is related to the yeast protein Rgr1, while other subunits like CRSP70 are similar to the elongation factor TFIIS, underscoring the evolutionary significance of these interactions. CRSP150 interacts with larger multimeric coactivator complexes, such as ARC/DRI, which directly binds to SREBP and nuclear hormone receptors to facilitate transcription, as well as NAT, a polymerase II-interacting complex that represses activated transcription. CRSP150′s multifaceted roles in transcriptional regulation make CRSP150 a vital target for research in gene expression and cellular function.

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CRSP150 Antibody (2214C1a) References:

1. A novel human SRB/MED-containing cofactor complex, SMCC, involved in transcription regulation.  |  Gu, W., et al. 1999. Mol Cell. 3: 97-108. PMID: 10024883
2. Competition between thyroid hormone receptor-associated protein (TRAP) 220 and transcriptional intermediary factor (TIF) 2 for binding to nuclear receptors. Implications for the recruitment of TRAP and p160 coactivator complexes.  |  Treuter, E., et al. 1999. J Biol Chem. 274: 6667-77. PMID: 10037764
3. Identity between TRAP and SMCC complexes indicates novel pathways for the function of nuclear receptors and diverse mammalian activators.  |  Ito, M., et al. 1999. Mol Cell. 3: 361-70. PMID: 10198638
4. Identification of mouse TRAP100: a transcriptional coregulatory factor for thyroid hormone and vitamin D receptors.  |  Zhang, J. and Fondell, JD. 1999. Mol Endocrinol. 13: 1130-40. PMID: 10406464
5. The structure of the nuclear hormone receptors.  |  Kumar, R. and Thompson, EB. 1999. Steroids. 64: 310-9. PMID: 10406480
6. The TRAP220 component of a thyroid hormone receptor- associated protein (TRAP) coactivator complex interacts directly with nuclear receptors in a ligand-dependent fashion.  |  Yuan, CX., et al. 1998. Proc Natl Acad Sci U S A. 95: 7939-44. PMID: 9653119
7. Mammalian mediator of transcriptional regulation and its possible role as an end-point of signal transduction pathways.  |  Jiang, YW., et al. 1998. Proc Natl Acad Sci U S A. 95: 8538-43. PMID: 9671713

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CRSP150 Antibody (2214C1a) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-CRSP150 Antibody (2214C1a) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CRSP150 Antibody (2214C1a) Specifications

Alternate Product Name: CRSP150

Product Clone Name: 2214C1a

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁

Amino Acids:

Epitope Target: CRSP150

Epitope Species: human

Product Reactivity/Detection: CRSP150 of human origin, CRSP2 of mouse origin, and the corresponding rat homolog

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 100 µg IgG₁ in 1.0 ml of PBS with < 0.1% sodium azide and 1.0% stabilizer protein

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for CRSP150 Antibody (2214C1a): N/A