CROP Double Nickase Plasmid (m): sc-426686-NIC
- Target species: mouse
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CROP Double Nickase Plasmid (m) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CROP Double Nickase Plasmid (m) and CROP Double Nickase Plasmid (m2) encode distinct paired gRNA designs targeting Luc7l3. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CROP Double Nickase Plasmid (m) | sc-426686-NIC | 20 µg | $410.00 |
Luc7l3 encodes the mouse CROP protein, an RNA-binding factor implicated in pre-mRNA splicing and spliceosome-associated regulation of transcript maturation. By influencing alternative splicing decisions and mRNA processing, Luc7l3 can affect downstream gene-expression programs involved in cell-state transitions, immune signaling, and differentiation. Perturbation of splicing regulators is broadly linked to transcriptome instability and altered proteome output, processes frequently studied in inflammation, hematopoiesis, and cancer-related dysregulation. Luc7l3 therefore serves as a useful node for investigating how spliceosomal control shapes pathway activity and phenotype in mammalian cells.
CROP Double Nickase Plasmid (m) consists of a matched pair of plasmids engineered for high-specificity editing of the Luc7l3 locus in mouse cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within Luc7l3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt Luc7l3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of Luc7l3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.