CROP Double Nickase Plasmid (h): sc-412243-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CROP Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CROP Double Nickase Plasmid (h) and CROP Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting LUC7L3. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CROP Double Nickase Plasmid (h) | sc-412243-NIC | 20 µg | $410.00 | |||
CROP Double Nickase Plasmid (h2) | sc-412243-NIC-2 | 20 µg | $410.00 |
LUC7L3 encodes CROP, an SR-related RNA-binding protein that contributes to spliceosome assembly and pre-mRNA splicing fidelity. CROP participates in co- and post-transcriptional RNA processing, influencing alternative exon usage and transcript stability in pathways linked to cell cycle control, stress responses, and differentiation. Dysregulated splicing programs involving LUC7L3 have been associated with altered gene expression networks observed across multiple disease-relevant contexts, including cancer biology and inflammatory signaling. As a human splicing factor, CROP is often studied to understand how perturbations in RNA processing reshape proteome output and cellular phenotypes.
CROP Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the LUC7L3 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within LUC7L3. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt LUC7L3 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of LUC7L3-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.