CPEB2 Double Nickase Plasmid (h): sc-409367-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- CPEB2 Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- CPEB2 Double Nickase Plasmid (h) and CPEB2 Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CPEB2. One or both designs may be available
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
CPEB2 Double Nickase Plasmid (h) | sc-409367-NIC | 20 µg | $410.00 | |||
CPEB2 Double Nickase Plasmid (h2) | sc-409367-NIC-2 | 20 µg | $410.00 |
CPEB2 (cytoplasmic polyadenylation element binding protein 2) is an RNA-binding protein that recognizes cytoplasmic polyadenylation elements in target mRNAs to regulate poly(A) tail length, translation, and mRNA stability. It contributes to post-transcriptional gene control programs involved in cell differentiation, stress responses, and context-dependent modulation of protein synthesis. Through selective translational regulation, CPEB2 influences pathways linked to cellular adaptation such as hypoxia-associated signaling and RNA granule dynamics, with downstream effects on proliferation and survival. Dysregulated CPEB2 activity and altered RNA metabolism have been investigated in relation to tumor biology and other diseases where aberrant translational control is a hallmark.
CPEB2 Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CPEB2 locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CPEB2. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CPEB2 function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CPEB2-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.