CPA2 Antibody (B-5): sc-393547

CPA2 Antibody (B-5) is a mouse monoclonal IgG1 kappa light chain antibody that detects CPA2 protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry, and enzyme-linked immunosorbent assay (ELISA). CPA2 (B-5) antibody is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. CPA2, or Carboxypeptidase A2, is a crucial enzyme in the digestive system, primarily involved in the hydrolysis of C-terminal amino acids from proteins, which is essential for protein digestion and nutrient absorption. As a member of the M14 metallocarboxypeptidase family, CPA2 exhibits distinct substrate specificity, favoring bulkier C-terminal residues compared to its closely related counterpart, CPA1. This specificity is vital for the proper processing of dietary proteins and the regulation of various physiological processes. CPA2 is predominantly expressed in the pancreas, brain, lung, and testis, highlighting its diverse roles in both digestive and neurological functions. The presence of a characteristic propeptide at the amino-terminus of CPA2, which is cleaved during enzyme activation, further underscores the importance of post-translational modifications in regulating its activity and function.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CPA2 Antibody (B-5) References:

1. Structure of a novel leech carboxypeptidase inhibitor determined free in solution and in complex with human carboxypeptidase A2.  |  Reverter, D., et al. 2000. Nat Struct Biol. 7: 322-8. PMID: 10742178
2. Structural evolution of an enzyme specificity. The structure of rat carboxypeptidase A2 at 1.9-A resolution.  |  Faming, Z., et al. 1991. J Biol Chem. 266: 24606-12. PMID: 1761558
3. Further studies on the human pancreatic binary complexes involving procarboxypeptidase A.  |  Moulard, M., et al. 1990. FEBS Lett. 261: 179-83. PMID: 2307232
4. A novel rat carboxypeptidase, CPA2: characterization, molecular cloning, and evolutionary implications on substrate specificity in the carboxypeptidase gene family.  |  Gardell, SJ., et al. 1988. J Biol Chem. 263: 17828-36. PMID: 3182871
5. Structural characterization of the rat carboxypeptidase A1 and B genes. Comparative analysis of the rat carboxypeptidase gene family.  |  Clauser, E., et al. 1988. J Biol Chem. 263: 17837-45. PMID: 3182872
6. Carboxypeptidase A isoforms produced by distinct genes or alternative splicing in brain and other extrapancreatic tissues.  |  Normant, E., et al. 1995. J Biol Chem. 270: 20543-9. PMID: 7657630
7. Separation of human pancreatic carboxypeptidase A isoenzymes by high performance liquid chromatography.  |  Linder, D., et al. 1993. Biomed Chromatogr. 7: 143-5. PMID: 8318831
8. Expression and characterization of human pancreatic preprocarboxypeptidase A1 and preprocarboxypeptidase A2.  |  Laethem, RM., et al. 1996. Arch Biochem Biophys. 332: 8-18. PMID: 8806703

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CPA2 Antibody (B-5) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-CPA2 Antibody (B-5) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CPA2 Antibody (B-5) Specifications

Alternate Product Name: CPA2

Product Clone Name: B-5

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₁ kappa light chain

Amino Acids: 1-56

Epitope Target: CPA2

Epitope Species: mouse

Product Reactivity/Detection: CPA2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for CPA2 Antibody (B-5): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin