Cox-1 (m): 293T Lysate: sc-126660

Cox-1 (m): 293T Lysate is derived from the 293T cell line, a human embryonic kidney cell line engineered to express the SV40 large T antigen, enhancing its transfection efficiency and protein production capabilities. This lysate is enriched with mouse Cyclooxygenase-1 (Cox-1), an enzyme that plays a crucial role in the biosynthesis of prostaglandins from arachidonic acid. Prostaglandins are lipid compounds that perform various physiological functions, including the regulation of inflammation, maintenance of the gastric mucosa, and platelet aggregation. In research, Cox-1 (m): 293T Lysate is utilized to study the enzymatic activity and regulatory mechanisms of Cox-1. Researchers employ this lysate in Western blotting to detect and quantify Cox-1 expression levels under different experimental conditions, providing insights into its regulation and functional roles. The lysate is also used in enzymatic activity assays to measure the conversion of arachidonic acid to prostaglandins, helping to explain the catalytic properties of Cox-1. Additionally, Cox-1 (m): 293T Lysate is valuable in co-immunoprecipitation assays to identify protein-protein interactions involving Cox-1, which aids in understanding its role within larger signaling networks. Structural and functional studies using this lysate can explore the conformational changes and substrate specificity of Cox-1, contributing to a deeper understanding of its physiological functions.