Cox-1 Antibody (6D317) recommended for detection of Cyclooxygenase-1 of mouse, rat, human and ovine origin by WB, IP, IF, IHC(P) and FCM

Cox-1 Antibody (6D317): sc-70877

Cox-1 Antibody (6D317) is rated 4.3 out of 5 by 4.
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Datasheets

    • Cox-1 Antibody (6D317) is a mouse monoclonal IgG2b κ, cited in 1 publications, provided at 200 µg/ml
    • raised against Cox-1 purified from ovine seminal vesicles
    • recommended for detection of Cyclooxygenase-1 of mouse, rat, human and ovine origin by WB, IP, IF, IHC(P) and FCM; may cross-react with human recombinant, human platelet, rat and mouse Cox-1, and slightly (5%) with ovine Cox-2; non cross-reactive with human or mouse Cox-2
    • See Cox-1 (11): sc-19998 for Cox-1 antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.

Can Cox-1 (6D317): sc-70877 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Germaine
Thank you for your question. Yes, Cox-1 (6D317): sc-70877 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from Works well for WB with NIH/3T3 whole cell lysate Works well for WB with NIH/3T3 whole cell lysate.. -SCBT QC
Date published: 2015-03-15
Rated 4 out of 5 by from Good specificity and signal strength with Good specificity and signal strength with some background bands -SCBT QC
Date published: 2015-02-22
Rated 5 out of 5 by from Strong band in WB when used on human Cox Strong band in WB when used on human Cox-1 transfected 293T and U-937 whole cell lysates. -SCBT QC
Date published: 2015-02-09
Rated 4 out of 5 by from Performs well in Western Performs well in Western, good signal with rat aortic smooth muscle cells. -SCBT Publication Review
Date published: 2013-07-02
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