Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Not CRISPR data: the image illustrates antibody quality for visualizing CRISPR experiment results.
Cortactin Double Nickase Plasmid (h): sc-400761-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- Cortactin Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- Cortactin Double Nickase Plasmid (h) and Cortactin Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting CTTN. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: Cortactin Antibody (H-5): sc-55579
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
Cortactin Double Nickase Plasmid (h) | sc-400761-NIC | 20 µg | $410.00 | |||
Cortactin Double Nickase Plasmid (h2) | sc-400761-NIC-2 | 20 µg | $410.00 |
CTTN encodes cortactin, an actin-binding scaffolding protein that coordinates Arp2/3-dependent actin branching and stabilizes dynamic cortical actin networks. By linking filament assembly with signaling modules, cortactin supports lamellipodia formation, endocytosis, and invadopodia maturation, integrating cues from Src family kinases and Rho GTPase pathways to regulate adhesion and motility. Altered CTTN dosage or phosphorylation status has been associated with dysregulated cytoskeletal remodeling and invasive phenotypes, making it relevant to studies of tumor cell migration and metastasis-associated signaling. Cortactin also contributes to membrane trafficking and receptor turnover, connecting actin dynamics to growth factor signaling outputs.
Cortactin Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the CTTN locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within CTTN. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt CTTN function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of CTTN-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.