COPA Double Nickase Plasmid (h): sc-404020-NIC
- Target species: human
- 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
- COPA Double Nickase Plasmid (h) consists of a pair of plasmids each encoding a D10A mutated Cas9 nuclease and a target-specific 20 nt guide RNA (gRNA) designed to knockout gene expression with greater specificity than its CRISPR/Cas9 KO counterpart
- Paired gRNA sequences are offset by approximately 20 bp to allow for specific Cas9-mediated double nicking of the genomic DNA, which mimics a DSB
- One plasmid in the pair contains a puromycin-resistance gene for selection; the other plasmid in the pair contains a GFP marker to visually confirm transfection
- COPA Double Nickase Plasmid (h) and COPA Double Nickase Plasmid (h2) encode distinct paired gRNA designs targeting COPA. One or both designs may be available
- Following transfection, gene knockout efficiency can be assayed by WB, IF or IHC using antibody: COPA Antibody (H-3): sc-398099
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
COPA Double Nickase Plasmid (h) | sc-404020-NIC | 20 µg | $410.00 | |||
COPA Double Nickase Plasmid (h2) | sc-404020-NIC-2 | 20 µg | $410.00 |
COPA encodes the alpha subunit of the COPI coatomer complex, a central regulator of retrograde vesicular trafficking from the Golgi apparatus to the endoplasmic reticulum and within the Golgi. By coordinating cargo selection and coat assembly, COPA supports protein quality control, lipid and membrane homeostasis, and ER stress responses that influence secretory pathway function. Perturbation of COPA-dependent trafficking can alter proteostasis and intracellular signaling, affecting processes such as autophagy, inflammasome activity, and antigen presentation. Variants in COPA have been linked to immune dysregulation and inflammatory phenotypes, making it a relevant target for mechanistic studies of vesicle transport and stress-associated signaling.
COPA Double Nickase Plasmid (h) consists of a matched pair of plasmids engineered for high-specificity editing of the COPA locus in human cell lines. Each plasmid expresses a Cas9 D10A nickase and a distinct sgRNA targeting opposite DNA strands within COPA. When directed to adjacent sites on opposite DNA strands, the two nickases generate offset single-strand nicks that together produce a staggered double-strand break, requiring coordinated on-target activity from both guides. The resulting DNA break is resolved by endogenous cellular repair pathways, most commonly through non-homologous end joining (NHEJ), leading to insertions or deletions that disrupt COPA function. By requiring dual sgRNA engagement at the target locus, the double nicking approach enhances editing specificity and provides a complementary CRISPR strategy for applications where additional control over targeting precision is desired.
To support efficient identification of edited cells, one plasmid encodes GFP for fluorescent visualization of transfected populations, while the companion plasmid carries a puromycin resistance gene for antibiotic selection. Together, these features support efficient enrichment of co-transfected populations and simplify the validation of COPA-disrupted clones.
For Research Use Only. Not Intended for Diagnostic or Therapeutic Use.