Control CRISPR/Cas9 Plasmid Control CRISPR/Cas9 Plasmid is a plasmid encoding the Cas9 nuclease and a non-specific 20 nt guide RNA. Used as a negative control for CRISPR/Cas9 KO Plasmids

Control CRISPR/Cas9 Plasmid

Control CRISPR/Cas9 Plasmid is rated 5.0 out of 5 by 2.
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Datasheets

    • 20 µg of transfection-ready, purified plasmid DNA; Suitable for up to 20 transfections
    • Control CRISPR/Cas9 Plasmid is a plasmid encoding the Cas9 nuclease and a non-specific 20 nt guide RNA. Used as a negative control for CRISPR/Cas9 KO Plasmids
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Does this plasmid or the CRISPR/Cas9 Knockout Plasmids carry antibiotic resistance gene for maxi-prep?

Asked by: Matrix
Thank you for your question. Our CRISPR/Cas9 KO Plasmids are provided as transfection-ready purified plasmid DNA. We do not recommend nor support further preparation of the plasmids. They are intended to be a consumable reagent.
Answered by: Technical Support
Date published: 2019-06-16

Hi, I have question about SC-418922 negative control. How long express GFP in the cells after transfection? I want expand cell upto 10miliion. If I expand cells after transfection, does GFP keep express or decrease with cell division ?

Asked by: park
Thank you for your question. The GFP is not integrated into the host cell genome, it is expressed transiently from the plasmid while it is present in the cell. It will decrease with cell division. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-11-18

How can I make a stable cell line with this control crispr-cas9 plasmid?

Asked by: Andrew Lai
Thank you for your question. This control plasmid will not knock down any known genes in your cells, so any cell line transfected with these control CRISPR plasmids should keep on living normally. Please contact Technical Service by phone, (800)-457-3801 option 2, email <[email protected]>, or by live chat directly on our website, www.scbt.com if you have any further questions.
Answered by: Tech Service
Date published: 2017-06-20
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Rated 5 out of 5 by from We are very satisfied with this product We are very satisfied with this product. We used the plasmid as control for knock-out of ATF-2 in human colorectal adenocarcinoma cell line Colo201 and had no effect on ATF-2.
Date published: 2015-10-09
Rated 5 out of 5 by from The CRISPR negative control demonstrated The CRISPR negative control demonstrated that the non-specific gRNAs did not effect E2F-1 and GAPDH expression in transfected HEK 293T whole cell lysates. -SCBT QC
Date published: 2015-03-24
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